Team:Hawaii/Notebook/2008-08-30

From 2008.igem.org

(Difference between revisions)
Gracek (Talk | contribs)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Construction of p+r and rereplacement of BB-pRL1383a MCS=== :<strong> Grace</strong> [[Image:083008REdigests.jpg|right|...)
Newer edit →

Revision as of 21:49, 30 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Construction of p+r and rereplacement of BB-pRL1383a MCS

Grace
EtBr stained 2% (L) and 0.8% (R) agarose gels ran at 60V for 2 hours and 1 hour, respectively. Thirty microliters of RE digest reaction were loaded into each well.
  • Ran RE digests from last night on agarose gel
  • Extracted bands from gel
  • Ligated:
  • BB-pRL1383a with J33207
  • nir + rbs (B0034)
  • plac + rbs (B0034)
  • RE digested p+r with EcoRI, XbaI, SpeI, PstI

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


Retrieved from "http://2008.igem.org/Team:Hawaii/Notebook/2008-08-30"