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Team:KULeuven/Primers
From 2008.igem.org
(Difference between revisions)
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|name=? | |name=? | ||
|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTC</div> | |sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTC</div> | ||
| - | |Tm= | + | |Tm=61.2 |
|length=23 | |length=23 | ||
| - | |comments= | + | |comments=Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling. |
}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
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|name=? | |name=? | ||
|sequence=<div class="code">CTGCAGCGGC CGCTACTAG</div> | |sequence=<div class="code">CTGCAGCGGC CGCTACTAG</div> | ||
| - | |Tm= | + | |Tm=60.9 |
|length=19 | |length=19 | ||
| - | |comments= | + | |comments=Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling. |
}} | }} | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
Revision as of 13:02, 1 September 2008
dock/undock dropdown
| Table of Oligos | |||||
|---|---|---|---|---|---|
| Number | Name | Sequence | Length | Tm (°C) | Comments |
| 1076 | T7 Fw, no tag | ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
| 42bp | 66.7 | Forward T7 RNApol primer, without UmuD tag |
| 1077 | T7 Rev | CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
| 44bp | 59.9 | Reverse T7 RNApol primer |
| 1078 | T7 Fw, UmuD tag, part 1 | CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
| 62bp | 51.0 | Forward T7 RNApol primer, with UmuD tag, part 1 |
| 1079 | T7 Tw, UmuD tag, part 2 | ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
| 91bp | 55.8 | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
| 1084 | GFP-LVA Fw | CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG
| 55bp | 58.9 | Forward GFP-LVA primer |
| 1085 | GFP-LVA Rev | CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC
| 64bp | 64.9 | Reverse GFP-LVA primer |
| 1074 | VF2 | TGCCACCTGA CGTCTAAGAA
| 20bp | 55.9 | Forward primer for sequence analysis |
| 1075 | VR | ATTACCGCCT TTGAGTGAGC
| 20bp | 55.3 | Reverse primer for sequence analysis |
| 1100 | antisense luxI Fw | CATCAGGAAT TCGCGGCCGC TTCTAGTTAT TAAGCTACTA AAGCGTAGTT TTC
| 53bp | 51.6 | Forward primer for construction of antisense luxI, contains prefix, contains additional CATCAG
|
| 1101 | antisense luxI Rev | CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGG
| 46bp | 47.8 | Reverse primer for construction of antisense luxI, contains suffix, 25 bp annealing |
| 1127 | ccdB coding Fw | CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC
| 47 | 50.9 | Forward primer for amplification of ccdB coding sequence, contains prefix, additional CATCAT
|
| 1128 | ccdB coding Rev | CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG
| 43 | 51.8 | Reverse primer for amplification of ccdB, contains suffix |
| 1129 | Hybrid promoter left linker | CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
| 65 | check | Left linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains prefix, additional CATCAT
|
| 1130 | Hybrid promoter right linker | TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG
| 64 | check | right linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains suffix |
| 1131 | BBa_I714070 lock Fw | CATCATGAAT TCGCGGCCGC TTCTAGAGTG TAGACCGAAC TAGAATCACC TC
| 52 | check | Forward primer for amplication of lock from BBa_I714070 (J23078), contains scar, contains prefix, additional CATCAT
|
| 1132 | BBa_I714070 lock Rev | CTGCAGCGGC CGCTACTAGT ATC
| 23 | 61.5 | Reverse primer for amplication of lock from BBa_I714070 (J23078), contains suffix |
| 1133 | BBa_I714074 key Fw | CATCATGAAT TCGCGGCCGC TTCTAGAGAC CCAAAAGCAA GAGGTG
| 46 | 57.3 | Forward primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains prefix, additional CATCAT
|
| 1134 | BBa_I714074 key Rev | CTGCAGCGGC CGCTACTAGT ATATAAAC
| 28 | 60.3 | Reverse primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains suffix |
| 1152 | ? | CATCATGAAT TCGCGGCCGC TTC
| 23 | 61.2 | Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling. |
| 1153 | ? | CTGCAGCGGC CGCTACTAG
| 19 | 60.9 | Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling. |
| 1156 | ? | CAAGCCTGCG GATCTCCGCG AAATTGTGAC TTTTCCGCTA TTTAGCGATC TTGTTCAG
| 58 | ? | ? |
| 1157 | ? | CATCATGAAT TCGCGGCCGC TTCTAGATGT TGTTTATCAA GCCTGCGGAT CTCC
| 54 | ? | ? |
