Team:KULeuven/Project/Reset

From 2008.igem.org

(Difference between revisions)

Revision as of 10:36, 2 September 2008

dock/undock dropdown

Reset

BioBricks

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 BBa_I712074]) followed by a RiboLock ([http://partsregistry.org/Part:BBa_J23078 BBa_J23078]). The aiiA gene ([http://partsregistry.org/Part:BBa_C0060 BBa_C0060]), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference])

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced.  It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.

Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.

@@ Line 13: / Line 13: @@
 ===Action===
-The reset mechanism is made to destroy all HSL at the time, when significant input re-emerges. Originally, a pulse of lactonase was thought to do the thing, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the concentration should be high enough and the device should work.
+This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter '''Timer'''].
+ When significant input (re-)emerges, lactonase is produced.  It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
+Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.
-The ''aiiA'' gene coding for lactonase is regulated by the output signal from the filter, so that background input signal does not result in timer reset.
 {{:Team:KULeuven/Tools/Components}}