Team:KULeuven/5 September 2008

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* We MiniPrepped a few ligations and part R1052.
* We MiniPrepped a few ligations and part R1052.
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* We continued the digestion of R0011+ B0032, K145001+B0015
+
* We continued the digestion of K145001+B0015 and put R0011+B0032, K145150, K145013 on gel.
* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.
* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.
* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx  
* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx  
-
* The PCR we did yesterday (transduction and T7) was put on gel, but they both failed.
+
* The PCR we did yesterday (transduction and T7) was put on gel, but they both were not correct.
* We tried again to do a PCR of the T7 polymerase with UmuD tag.
* We tried again to do a PCR of the T7 polymerase with UmuD tag.
* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015
* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015

Revision as of 19:35, 5 September 2008

  dock/undock dropdown  

Contents

Lab Work

Wet Lab

Succes in the Wet Lab: we constructed the OUTPUT-subsystem with GFP with and without LVA-tag, as can be seen in this picture. On the left side GFP with LVA tag (our part K145015): less fluorescent. On the right side GFP without LVA tag (part E0042): more fluorescent.

DSC02957.JPG

Final Scheme till so far:

5sept final.PNG

Parallel Scheme till so far:

5sept parallel.PNG


NOT THAT MUCH MORE PARTS TO FINISH!!!!

  • We MiniPrepped a few ligations and part R1052.
  • We continued the digestion of K145001+B0015 and put R0011+B0032, K145150, K145013 on gel.
  • We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with EcoRI and SpeI; C0061+B0015, C0056+B0015, R0040+E0240 with XbaI). Only R1052 didn't cut well.
  • A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx
  • The PCR we did yesterday (transduction and T7) was put on gel, but they both were not correct.
  • We tried again to do a PCR of the T7 polymerase with UmuD tag.
  • We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015

Dry Lab

Dr. Coli and his danger, ethics, ethics, ethics...

Modeling

SimBiology2Latex Toolbox has been finalized and can be found on the wiki.

MultiCell Toolbox has entered it's final design stages. A preview of the GUI can be found on the wiki.

Some more work on diffusion has been done. Sensitivity Analyses is still a pain in the ***.

Wiki

Homepage has been revamped, removing a lot of bugs. IE fixes still need to follow. Components bar has been fixed.

Remarks

Strip of the day

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