Team:KULeuven/Ligation

From 2008.igem.org

(Difference between revisions)
m
(→Ligations: step 1)
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{{:Team:KULeuven/Tools/Header}}
{{:Team:KULeuven/Tools/Header}}
-
== Ligations: step 1 ==
+
== New Parts ==
-
{|
+
 
-
|- style="background: #003E81;color:white;width=100%"
+
{| style="background:#99CCFF; color:black; width:100%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
! colspan="6" style="background: #003E81" | Input
+
! BioBrick !! Description !! Status !! Experience
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=200| '''Name'''
+
| [http://partsregistry.org/Part:BBa_K145001 K145001] || T7 DNA polymerase (no tag)|| [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=160| '''Miniprep'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Digest'''
+
| [http://partsregistry.org/Part:BBa_K145013 K145013] || antisense LuxI|| electroporation || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Ligation'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Colonies'''
+
| [http://partsregistry.org/Part:BBa_K145014 K145014] || T7 DNA polymerase with UmuD tag|| [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"  
+
| [http://partsregistry.org/Part:BBa_K145015 K145015] || GFP with LVA tag|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
|J23116+B0032||ok||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145150 K145150] || hybrid promoter|| electroporation || [[Image:wrong.JPG|center|30px]]
-
|C0040+B0015||ok||ok||-||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145151 K145151] || ccdB coding region || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|R0040+E0240||-||-||-||-||-
+
|}
-
|- style="background: #003E81;color:white;width=100%"
+
 
-
! colspan="6" style="background: #003E81" | Output
+
 
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
==New Devices ==
-
|width=200| '''Name'''
+
 
-
|width=160| '''Miniprep'''
+
{| style="background:#99CCFF; color:black; width:100%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
|width=150| '''Digest'''
+
! BioBrick !! Components !! Description !! Status !! Experience
-
|width=150| '''Ligation'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Colonies'''
+
| [http://partsregistry.org/Part:BBa_K145201 K145201] || J23116+B0032+C0040+B0015 || Input || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145205 K145205] || R0040+B0032+K145015+B0015 || Output (LVA tag)|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| R0084+B0032||ok||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145252 K145252] || R0040+E0240 || Output (no tag) || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| E0022+B0015||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145280 K145280] || K145201+K145205 || Input-Output: test || ligation mix || [[Image:wrong.JPG|center|30px]]
-
| K145015+B0015||pcr||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145279 K145279] || K145201+K145252 || Input-Output: test || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
| E0040+B0015||-||-||-||-||-
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145258 K145258] || R0040+B0033+C0056+B0015 || Memory - part 1 || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
! colspan="6" style="background: #003E81" | Memory
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
| [http://partsregistry.org/Part:BBa_K145259 K145259] || R0053+P0152+R1052+P0353 || Memory - part 2 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=200| '''Name'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=160| '''Miniprep'''
+
| [http://partsregistry.org/Part:BBa_K145210 K145210] || K145258+K145259 || Memory || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Digest'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Ligation'''
+
| [http://partsregistry.org/Part:BBa_K145211 K145211] || R1052+K145013+B0015 || Memory - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Colonies'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Confirmed'''
+
| [http://partsregistry.org/Part:BBa_K145215 K145215] || R0040+J23066 || Filter - part 1 || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| R0053+P0152 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145278 K145278] || J23109+J23032+K145001+B0015 || Filter (old) - part 2 || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| R1052+P0353 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145277 K145277] || J23109+J23078+K145001+B0015 || Filter (new) - part 2 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| R0084+B0033 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145275 K145275] || K145215+K145278 || Filter (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| C0056+B0015 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145276 K145276] || K145215+K145277 || Filter (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| R1052+K145013 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145253 K145253] || I712074+J23032+C0012+B0015 || Invertimer (old) - part 1 || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| K145013+B0015 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145255 K145255] || I712074+J23078+C0012+B0015 || Invertimer (new) - part 1 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #003E81;color:white;width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
! colspan="6" style="background: #003E81" | Filter
+
| [http://partsregistry.org/Part:BBa_K145254 K145254] || R0011+B0032+C0061+B0015 || Invertimer - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=200| '''Name'''
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| [http://partsregistry.org/Part:BBa_K145220 K145220] || K145255+K145254 || Invertimer (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=160| '''Miniprep'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Digest'''
+
| [http://partsregistry.org/Part:BBa_K145104 K145104] || K145253+K145254 || Invertimer (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Ligation'''
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|width=150| '''Colonies'''
+
| [http://partsregistry.org/Part:BBa_K145251 K145251] || I712074+J23032+C0060+B0015 || Reset (old) || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145225 K145225] || I712074+J23078+C0060+B0015 || Reset (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| R0084+J23022 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145256 K145256] || K145150+B0034+C0062 || Cell Death - part 1 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| J23109+J23032 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145257 K145257] || B0014+B0033+K145151+B0015 || Cell Death - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| K145014+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145230 K145230] || K145256+K145257 || Cell Death || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| K145001+B0015 ||pcr||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145273 K145273] || R0040+P0353 || test K145150 - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| I7122074+J23032 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145270 K145270] || J23116+B0032+C0062+B0015 || test K145150 - part 3 || ligation mix || [[Image:wrong.JPG|center|30px]]
-
| K145015+B0015 ||pcr||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145269 K145269] || K145150+E0240 || test K145150 - part 4 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| E0040+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145268 K145268] || K145201+K145273 || test K145150 - part 5 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
! colspan="6" style="background: #003E81" | Inverter
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
| [http://partsregistry.org/Part:BBa_K145267 K145267] || K145270+K145269 || test K145150 - part 6 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=200| '''Name'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=160| '''Miniprep'''
+
| [http://partsregistry.org/Part:BBa_K145266 K145266] || K145268+K145267 || test K145150|| [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Digest'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Ligation'''
+
| [http://partsregistry.org/Part:BBa_K145271 K145271] || I712074+J23078+E0040+B0015 || test filter (new) - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Colonies'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Confirmed'''
+
| [http://partsregistry.org/Part:BBa_K145272 K145272] || I712074+J23032+E0040+B0015 || test filter (old) - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| I712074+J23032||ok||ok||ok||ok||ok
+
| [http://partsregistry.org/Part:BBa_K145265 K145265] || K145275+K145272 || test filter (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| C0012+B0015||ok||ok||ok||ok||-
+
| [http://partsregistry.org/Part:BBa_K145264 K145264] || K145276+K145271 || test filter (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0011+F1610||ok||-||-||-||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0011+E0240||-||-||-||-||-
+
-
|- style="background: #003E81;color:white;width=100%"
+
-
! colspan="6" style="background: #003E81" | Reset
+
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| I712074+J023022||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0060+B0015||ok||ok||ok||ok||-
+
-
|- style="background: #003E81;color:white;width=100%"
+
-
! colspan="6" style="background: #003E81" | Cell Death
+
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| J23100+B0032||ok||ok||ok||ok||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0062+B0015||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+B0032||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| P1010+B0015||-||-||-||-||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+E0240||-||-||-||-||-
+
|}
|}

Revision as of 00:23, 8 September 2008

  dock/undock dropdown  

New Parts

BioBrick Description Status Experience
[http://partsregistry.org/Part:BBa_K145001 K145001] T7 DNA polymerase (no tag)
Correct.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145013 K145013] antisense LuxI electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145014 K145014] T7 DNA polymerase with UmuD tag
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145015 K145015] GFP with LVA tag
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145150 K145150] hybrid promoter electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145151 K145151] ccdB coding region
Correct.JPG
Wrong.JPG


New Devices

BioBrick Components Description Status Experience
[http://partsregistry.org/Part:BBa_K145201 K145201] J23116+B0032+C0040+B0015 Input
Correct.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145205 K145205] R0040+B0032+K145015+B0015 Output (LVA tag)
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145252 K145252] R0040+E0240 Output (no tag)
Correct.JPG
Correct.JPG
[http://partsregistry.org/Part:BBa_K145280 K145280] K145201+K145205 Input-Output: test ligation mix
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145279 K145279] K145201+K145252 Input-Output: test ligation mix
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145258 K145258] R0040+B0033+C0056+B0015 Memory - part 1 ligation mix
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145259 K145259] R0053+P0152+R1052+P0353 Memory - part 2
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145210 K145210] K145258+K145259 Memory
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145211 K145211] R1052+K145013+B0015 Memory - part 3
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145215 K145215] R0040+J23066 Filter - part 1
Correct.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145278 K145278] J23109+J23032+K145001+B0015 Filter (old) - part 2 ligation mix
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145277 K145277] J23109+J23078+K145001+B0015 Filter (new) - part 2
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145275 K145275] K145215+K145278 Filter (old)
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145276 K145276] K145215+K145277 Filter (new)
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145253 K145253] I712074+J23032+C0012+B0015 Invertimer (old) - part 1
Correct.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145255 K145255] I712074+J23078+C0012+B0015 Invertimer (new) - part 1
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145254 K145254] R0011+B0032+C0061+B0015 Invertimer - part 2 electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145220 K145220] K145255+K145254 Invertimer (new)
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145104 K145104] K145253+K145254 Invertimer (old)
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145251 K145251] I712074+J23032+C0060+B0015 Reset (old)
Correct.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145225 K145225] I712074+J23078+C0060+B0015 Reset (new)
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145256 K145256] K145150+B0034+C0062 Cell Death - part 1
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145257 K145257] B0014+B0033+K145151+B0015 Cell Death - part 2 electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145230 K145230] K145256+K145257 Cell Death
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145273 K145273] R0040+P0353 test K145150 - part 2 electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145270 K145270] J23116+B0032+C0062+B0015 test K145150 - part 3 ligation mix
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145269 K145269] K145150+E0240 test K145150 - part 4 electroporation
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145268 K145268] K145201+K145273 test K145150 - part 5
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145267 K145267] K145270+K145269 test K145150 - part 6
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145266 K145266] K145268+K145267 test K145150
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145271 K145271] I712074+J23078+E0040+B0015 test filter (new) - part 3
Wrong.JPG
Wrong.JPG
[http://partsregistry.org/Part:BBa_K145272 K145272] I712074+J23032+E0040+B0015 test filter (old) - part 3
Wrong.JPG
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[http://partsregistry.org/Part:BBa_K145265 K145265] K145275+K145272 test filter (old)
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[http://partsregistry.org/Part:BBa_K145264 K145264] K145276+K145271 test filter (new)
Wrong.JPG
Wrong.JPG
Retrieved from "http://2008.igem.org/Team:KULeuven/Ligation"