"
Rensselaer/8 September 2008
From 2008.igem.org
(Difference between revisions)
Pooret (Talk | contribs)
(New page: '''DNA purification digest''' Fe(13ug) Plasmid(11ug) DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 ...)
Newer edit →
(New page: '''DNA purification digest''' Fe(13ug) Plasmid(11ug) DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 ...)
Newer edit →
Revision as of 20:49, 8 September 2008
DNA purification digest
Fe(13ug) Plasmid(11ug)
DNA 280ul 95ul XbaI 10 10 SprI 20 20 Buffer2 40 17.5 BSA 4 1.75 dH2O 46 30.75
____ _____
400 ul 175 ul
Insert checks digest
Fe-mRFP (~1ug)
DNA(30ug/ul) 33 MsCI(3U/ul) 6.7 EcoRI(2U/ul) 1 EcoBuff 5 BSA 0.5 dH2O 3.8
_____
50 ul
