"
Team:KULeuven/10 September 2008
From 2008.igem.org
(Difference between revisions)
(→Wet Lab) |
|||
| Line 4: | Line 4: | ||
=== Wet Lab === | === Wet Lab === | ||
| + | |||
| + | * We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with ''EcoR''I and ''Spe''I. | ||
| + | * We also made a glycerolstock of these ligations. | ||
| + | * The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration. | ||
| + | * We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2). | ||
| + | * We cut B0034 and J23109+J23032 with ''Eco''RI and ''Xba''I. | ||
=== Dry Lab === | === Dry Lab === | ||
Revision as of 21:50, 10 September 2008
dock/undock dropdown
Contents |
Lab Work
Wet Lab
- We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with EcoRI and SpeI.
- We also made a glycerolstock of these ligations.
- The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration.
- We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).
- We cut B0034 and J23109+J23032 with EcoRI and XbaI.
Dry Lab
Blending the ethics...
Modeling
Wiki
Remarks
FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!!
| << return to notebook | return to homepage >> | ||
| < previous friday | ← yesterday | tomorrow → | next monday > |
