Team:KULeuven/10 September 2008

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* We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).  
* We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).  
* We cut B0034 and J23109+J23032 with ''Eco''RI and ''Xba''I.
* We cut B0034 and J23109+J23032 with ''Eco''RI and ''Xba''I.
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* We made a liquid culture of K145279 and K145280 to test the relation input/output with anhydrotetracycline.
=== Dry Lab ===
=== Dry Lab ===

Revision as of 22:03, 10 September 2008

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Contents

Lab Work

Wet Lab

  • We MiniPrepped the ligations that succeeded yesterday: K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015. Then K145258 was cut with EcoRI and SpeI.
  • We also made a glycerolstock of these ligations.
  • The digests we made yesterday were put on gel. Then we extracted them and put again 2ul on gel to estimate the concentration.
  • We ligated the following parts: K145201 + (R0040+P0353, C0062 (Pst) + (K145150+B0034, K145253 + K145254, (I712074+J23032) + (E0040+B0015), (B0014+B0033) + (K145151+B0015), B0015 (Pst) + (K145013+pSB1A2).
  • We cut B0034 and J23109+J23032 with EcoRI and XbaI.
  • We made a liquid culture of K145279 and K145280 to test the relation input/output with anhydrotetracycline.

Dry Lab

Blending the ethics...

Modeling

Wiki

Remarks

FC André had an important match today, the final score was 8-8! We were there and it was clear our team was far better than the others! They diserved to win!!!!

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