"
Team:Chiba/protocol/ligation/dephosphorylation
From 2008.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
#Incubate at 37℃ for 15~30 min. | #Incubate at 37℃ for 15~30 min. | ||
#Incubate at 65℃ for 15 min. (for inactivation by heat treatment) | #Incubate at 65℃ for 15 min. (for inactivation by heat treatment) | ||
| - | #Purify the DNA with Zymo | + | #Purify the DNA with Zymo DNA Cleam&Concentrator Kit |
READ:[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo cleam]] | READ:[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo cleam]] | ||
Latest revision as of 03:09, 11 September 2008
dephosphorylation
SAP: Alkaline Phosphatase (Shrimp)
- Mix
DNA fragment 1~10 pmol Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U 10X SAP Buffer 5 μ l Sterilized distilled water up to 50 μ l
- Incubate at 37℃ for 15~30 min.
- Incubate at 65℃ for 15 min. (for inactivation by heat treatment)
- Purify the DNA with Zymo DNA Cleam&Concentrator Kit
READ:Zymo cleam
