Latest revision as of 20:16, 15 September 2008

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Things we did today

Margaret

Gel of saturday's PCR.

Rep, oriV- correct size, so restriction digest with X,P of PCR product
omega- correct size, but still has a ladder, so re-digest of plasmid prep with S, X
oriT did not show up, but I found a re-digest from 7/24 that I will use for further cloning
positive control did not work, not sure if negative control worked or if I did something wrong.

Grace

Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 11: / Line 11: @@
 :* positive control did not work, not sure if negative control worked or if I did something wrong.
-== Drylab Work ==
-===Name of Task===
+===Plasmid prep===
-:<strong> name of person/people who performed the task</strong>
+:<strong>Grace</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+:* Inoculated TB+amp<sub>100</sub> with:
-:* e.g. read through papers, worked on proposal, etc.
+::* 3A: nir+rbs+slr1 #2, 3
+::* 3A: nir+rbs+pilA #6
+::* 3A: nir+rbs+slr1+GFPf #10
+::* 3A: nir+rbs+pilA+GFPf12 # 2, 6
+::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
+:* Inoculated TB+sp<sub>100</sub>
+::* BB-pRL1383a
+===Sequencing===
+:<strong>Grace</strong>
+:* ExoSAP'd, determined DNA conc., prep'd for sequencing:
+::* 3A: nir+rbs+slr1 #2, 3
+::* 3A: nir+rbs+pilA #6
+::* 3A: nir+rbs+slr1+GFPf #10
+::* 3A: nir+rbs+pilA+GFPf12 # 2, 6
+::* 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
+::* plac+rbs+slr1+GFPf #8
+:* Sent in samples from today and from last week for sequencing by CORE Hawaii
 = Discussion =