"
Team:Hawaii/Notebook/2008-09-16
From 2008.igem.org
(Difference between revisions)
(→Drylab Work) |
(→Gel Verify constructs) |
||
| Line 15: | Line 15: | ||
===Gel Verify constructs=== | ===Gel Verify constructs=== | ||
| + | |||
| + | [[Image:gel_veri_9_16.jpg|right|thumb|200px|Gel used to verify the insertion of Promoter and RBS in front of aada(prl&bb).]] | ||
:<strong>Margaret</strong> | :<strong>Margaret</strong> | ||
| - | |||
| - | |||
:*verified constructs: ap36, ab92, ab94, ab95, ab96, ab98 | :*verified constructs: ap36, ab92, ab94, ab95, ab96, ab98 | ||
:*verified constructs will be gel purified and used in a ligation. | :*verified constructs will be gel purified and used in a ligation. | ||
| - | |||
| - | |||
| - | |||
= Discussion = | = Discussion = | ||
Revision as of 06:25, 18 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Grace
- 5 ml plasmid prep of:
- BB-pRL1383a
- 3A: nir+rbs+slr1 #2, 3
- 3A: nir+rbs+pilA #6
- 3A: nir+rbs+slr1+GFPf #10
- 3A: nir+rbs+pilA+GFPf12 # 2, 6
- 3A: nir+rbs+pilA+GFPf13 # 4, 6, 8, 10
Gel Verify constructs
- Margaret
- verified constructs: ap36, ab92, ab94, ab95, ab96, ab98
- verified constructs will be gel purified and used in a ligation.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
