Team:UNIPV-Pavia/Notebook/Week17
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*We prepared 0.5 l of LB + Amp for liquid cultures. | *We prepared 0.5 l of LB + Amp for liquid cultures. | ||
- | *We infected 9 ml of LB + Amp with 30 | + | *We infected 9 ml of LB + Amp with 30 µl of 30(3), 31, 22, 13 and two falcon tubes for "a". |
+ | |||
+ | *We received 3OC6HSL from Sigma! we resuspended it in ddH2O, we prepared some stocks and stored them at -20°C. | ||
+ | |||
+ | '''09/9/08''' | ||
+ | <br> | ||
+ | *Glycerol stocks/miniprep for 30(3), 31, 22 and 13. | ||
+ | |||
+ | *Plasmid digestion for: | ||
+ | **30 (S-P) | ||
+ | **31 (S-P) | ||
+ | **30 (X-P) | ||
+ | **22 (X-P) | ||
+ | **13 (X-P) | ||
+ | |||
+ | *Run/gel extraction. | ||
+ | |||
+ | *Ligations: | ||
+ | **31-30 (="34") | ||
+ | **31-22 (="35") | ||
+ | **30-13 (for green fluorescence test) | ||
+ | |||
+ | *We induced |
Revision as of 22:29, 22 September 2008
Home | The Team | The Project | Biological Safety | Parts Submitted to the Registry |
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Notebook
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
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Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
Week 16: 09/8/08 - 09/12/08
09/8/08
- We prepared 0.5 l of LB + Amp for liquid cultures.
- We infected 9 ml of LB + Amp with 30 µl of 30(3), 31, 22, 13 and two falcon tubes for "a".
- We received 3OC6HSL from Sigma! we resuspended it in ddH2O, we prepared some stocks and stored them at -20°C.
09/9/08
- Glycerol stocks/miniprep for 30(3), 31, 22 and 13.
- Plasmid digestion for:
- 30 (S-P)
- 31 (S-P)
- 30 (X-P)
- 22 (X-P)
- 13 (X-P)
- Run/gel extraction.
- Ligations:
- 31-30 (="34")
- 31-22 (="35")
- 30-13 (for green fluorescence test)
- We induced