"
Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
m (→Miniprep (stored in the fridge in the lab at 8°C)) |
m (→Digests (stored in the fridge in the lab at 8°C)) |
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| Line 422: | Line 422: | ||
|[http://partsregistry.org/Part:BBa_K145150 K145150]|| ''Eco''R I||''Spe'' I||Insert||110||+/-25||9.2||54||Sept 05 | |[http://partsregistry.org/Part:BBa_K145150 K145150]|| ''Eco''R I||''Spe'' I||Insert||110||+/-25||9.2||54||Sept 05 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_K145151 K145151]|| ''Xba'' I||/||Vector||2400||28|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]|| ''Xba'' I||/||Vector||2400||28||-||57||August 12 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | |[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2732||+/-8||24.3||48||August 12 | ||
| Line 515: | Line 515: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||101||+/-30||4.0||B3||August 14 | |[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||101||+/-30||4.0||B3||August 14 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0034 B0034]|| ''Eco''R I||''Spe'' I||Insert||68||+/-30||3.0||F4||Sept 19 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_K145201 K145201]|| ''Eco''R I||''Spe'' I||Insert||900||+/-30||6.1||F5||Sept 19 | ||
| + | |- style="background: #E0FFFF; text-align: center;" | ||
| + | |[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||4080||+/-30||20.2||F6||Sept 19 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2986||+/-25||17.8||B4||Sept 03 | |[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2986||+/-25||17.8||B4||Sept 03 | ||
| Line 539: | Line 545: | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145251 K145251]|| ''Eco''R I||''xba'' I||Vector||3133||+/-20||21.0||D4||Sept 07 | |[http://partsregistry.org/Part:BBa_K145251 K145251]|| ''Eco''R I||''xba'' I||Vector||3133||+/-20||21.0||D4||Sept 07 | ||
| - | |||
| - | |||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_K145254 K145254]|| ''Eco''R I||''Xba'' I||Vector||2925||+/-30|| | + | |[http://partsregistry.org/Part:BBa_K145254 K145254]|| ''Eco''R I||''Xba'' I||Vector||2925||+/-30||-||D7||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_K145254 K145254]|| ''Eco''R I||''Xba'' I||Vector||2925||+/-30||23.9||E9||Sept 14 | |[http://partsregistry.org/Part:BBa_K145254 K145254]|| ''Eco''R I||''Xba'' I||Vector||2925||+/-30||23.9||E9||Sept 14 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2505||+/-30|| | + | |[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2505||+/-30||-||D9||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||3004||+/-30|| | + | |[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 E0240]|| ''Eco''R I||''Xba'' I||Vector||3004||+/-30||-||E1||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Spe'' I||''Pst'' I||Vector||2682||+/-30|| | + | |[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Spe'' I||''Pst'' I||Vector||2682||+/-30||-||E2||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3327||+/-25||18.1||B8||Sept 03 | |[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||3327||+/-25||18.1||B8||Sept 03 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_E0040 E0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2919||+/-30|| | + | |[http://partsregistry.org/Part:BBa_E0040 E0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]|| ''Eco''R I||''Xba'' I||Vector||2919||+/-30||-||D8||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||B9||August 25 | |[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||71||+/-28||2.5||B9||August 25 | ||
| Line 564: | Line 568: | ||
|[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||C3||August 25 | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-28||2.6||C3||August 25 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353]|| ''Eco''R I||''Xba'' I||Vector||4100||+/-30|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353]|| ''Eco''R I||''Xba'' I||Vector||4100||+/-30||-||D6||Sept 10 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-25||3.8||C4||Sept 05 | |[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||90||+/-25||3.8||C4||Sept 05 | ||
| Line 574: | Line 578: | ||
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||365||+/-20||6.9||C7||August 14 | |[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]|| ''Eco''R I||''Spe'' I||Insert||365||+/-20||6.9||C7||August 14 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Pst'' I||''Spe'' I||Vector||3468||+/-30||7.9|| | + | |[http://partsregistry.org/Part:BBa_I712074 I712074]|| ''Pst'' I||''Spe'' I||Vector||3468||+/-30||7.9||E5||Sept 13 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||500||+/-30||6.9|| | + | |[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||500||+/-30||6.9||E6||Sept 13 |
|} | |} | ||
Revision as of 11:18, 26 September 2008
dock/undock dropdown
Miniprep (stored in the fridge in the lab at 8°C)
| MiniPrep Products: Parts |
|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 10 | 112.3 | 7 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 20 | 130.3 | 8 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 303.1 | 9 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 20 | 213.6 | 10 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 48 | 311.0 | 11 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 30 | 214.0 | 12 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 209.5 | 13 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 63.0 | 6 | July 23 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 5 | 51.0 | 57 | August 11 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 20 | 47.2 | 55 | August 27 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 40 | 27.0 | 56 | August 27 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1AK3 | RBS | +/- 50 | 36.5 | 43 | August 27 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1AK3 | RBS | +/- 23 | 38.4 | 44 | August 27 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 47 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 48 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 49 | July 29 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1AK3 | RBS | +/- 15 | 57 | 50 | August 27 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1AK3 | RBS | +/- 50 | 29.6 | 51 | August 27 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI | +/- 30 | 133.1 | 20 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 22 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 21 | July 29 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 20 | 177.1 | 4 | July 24 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 5 | July 24 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | pSB1A2 | lactonase production | +/- 48 | 84.2 | 53 | August 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1A2 | LuxR repressor | +/- 48 | 76.2 | 38 | August 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1AK3 | LuxR repressor | +/- 25 | 41.5 | 36 | August 27 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 50 | 57.6 | 16 | August 12 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 40 | 53.1 | 17 | August 12 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 50 | 41.4 | 18 | August 27 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | HSL Sender Device | +/- 20 | 31.5 | 19 | August 27 |
| [http://partsregistry.org/Part:BBa_I712022 I712022] | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 32 | July 29 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 20.5 | 33 | August 27 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 63.9 | 34 | August 27 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 26 | July 29 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 27 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 25 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 28 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 99.2 | 30 | August 27 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 69.2 | 31 | August 27 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 29 | July 29 |
| [http://partsregistry.org/Part:BBa_J23116 J23116] | BBa_J61002 (Ap) | Constitutive promoter | +/- 48 | 328.8 | 52 | August 08 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 42 | July 29 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1AK3 | LacI regulated promoter | +/- 50 | 30.8 | 40 | August 27 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | pSB1A2 | tetR repressible promoter | +/- 48 | 37.9 | 46 | August 11 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 3 | July 31 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 14 | July 23 |
| [http://partsregistry.org/Part:BBa_R1052 R1052] | pSB1K0 | 434cI regulated promoter | +/- 15 | 27.5 | 60 | Sept 05 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | pSB1A2 | Lactonase | 30 | 155.3 | 54 | July 31 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1A2 | LuxR repressor/activator | ? | 159.4 | 35 | July 31 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | lacI + pL promoter | ? | 68.0 | 39 | July 31 |
| [http://partsregistry.org/Part:BBa_J23066 J23066] | J23006 | ribokey | +/- 45 | 541 | 58 | August 30 |
| [http://partsregistry.org/Part:BBa_P0353 P0353] | pSB1AK3 | cII p22 van memory | +/- 45 | 406.9 | 1 | August 30 |
| [http://partsregistry.org/Part:BBa_P0152 P0152] | pSB1A2 | cI 434 van memory | +/- 44 | 265.4 | 2 | August 30 |
| [http://partsregistry.org/Part:BBa_E0240 E0240] | pSB1A2 | GFP generator | +/- 44 | 281 | 59 | August 30 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | pSB1A2 | cI represor | +/- 45 | 419.8 | 64 | August 30 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | pSB1A2 | autoinducer ASL synthetase, LuxI | +/- 45 | 338.6 | 65 | August 30 |
| [http://partsregistry.org/Part:BBa_B0014 B0014] | pSB1AK3 | terminator | +/- 45 | 344.4 | 66 | August 30 |
| [http://partsregistry.org/Part:BBa_E0040 E0040] | pSB1A2 | GFP | +/- 48 | 305.8 | 67 | August 30 |
| [http://partsregistry.org/Part:BBa_R0053 R0053] | pSB1A2 | p22 cII regulated promotor | +/- 25 | 72.1 | 69 | August 30 |
| [http://partsregistry.org/Part:BBa_I714074 I714074] | pSB1A2 | ribokey | +/- 48 | 469 | 68 | August 30 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | pSB1A2 | tetR repressible promotor | +/- 27 | 74.6 | 45 | Sept 01 |
| MiniPrep Products: Ligations |
|---|
| Parts | Plasmid | Description | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Cell Death | +/- 46 | 124.8 | A1 | August 08 |
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Cell Death | +/- 46 | 159.2 | H9 | Sept 16 |
| [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Memory | +/- 46 | 276.7 | D5 | Sept 05 |
| [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 46 | 159.1 | D6 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240] | pSB1A2 | Ligation for Output | +/- 46 | 281.9 | D7 | Sept 05 |
| [http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1A2 | Ligation for Cell Death | +/- 46 | 118.1 | D8 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066] | J23006 (Ap) | Ligation for Filter (new) | +/- 46 | 421.9 | D9 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033] | pSB1A2 | Ligation for Memory | +/- 46 | 81.2 | E1 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152] | pSB1A2 | Ligation for Memory | +/- 46 | 109.5 | E2 | Sept 05 |
| [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Output | +/- 46 | 151.8 | A5 | August 08 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 89.9 | A3 | August 08 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23078 J23078] | BBa_J23006 (Ap) | Ligation for Reset | +/- 46 | 159.2 | H8 | Sept 16 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 94.4 | A4 | August 08 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23078 J23078] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 141.3 | I1 | Sept 17 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Input | +/- 40 | 102.5 | A8 | August 25 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Input | +/- 35 | 96.2 | B2 | August 25 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP with LVA tag | +/- 46 | 152.7 | A2 | August 08 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP+LVA | +/- 48 | 257 | E4 | August 30 |
| [http://partsregistry.org/Part:BBa_K145253 K145253]+[http://partsregistry.org/Part:K145254 K145254] | pSB1A2 | Invertimer | +/- 45 | 242.1 | I2 | Sept 17 |
| [http://partsregistry.org/Part:BBa_K145253 K145253]+[http://partsregistry.org/Part:K145254 K145254] | pSB1A2 | Invertimer | +/- 45 | 209.7 | I3 | Sept 17 |
| [http://partsregistry.org/Part:BBa_K145014 K145014]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | T7 polymerase with UmuD | +/- 45 | 240.0 | I4 | Sept 17 |
| [http://partsregistry.org/Part:BBa_K145014 K145014]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | T7 polymerase with UmuD | +/- 45 | 288.7 | I5 | Sept 17 |
| [http://partsregistry.org/Part:BBa_K145202 K145202] | pSB1A2 | Input (complete) | +/- 45 | 203.9 | I6 | Sept 26 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | pSB1A2 | Input (complete) | +/- 45 | 221.3 | I7 | Sept 26 |
| [http://partsregistry.org/Part:BBa_K145270 K145270] | pSB1A2 | Test K145150 | +/- 45 | 233.4 | I8 | Sept 26 |
| [http://partsregistry.org/Part:BBa_K145201 K145270] | pSB1A2 | Test K145150 | +/- 45 | 228.9 | I9 | Sept 26 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB | +/- 48 | 204.6 | E3 | August 30 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | T7 without UmuD | +/- 48 | 414.8 | E5 | August 30 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB | +/- 48 | 188.4 | E6 | August 30 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 57.8 | B5 | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 72.1 | B8 | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 18.5 | B7 | August 28 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | ccdB coding region | +/- 46 | 23.3 | B6 | August 28 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP with LVA tag | +/- 20 | 104.5 | F6 | August 08 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for output | +/- 30 | 64.4 | A9 | August 25 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Output | +/- 35 | 93.1 | B1 | August 25 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 81.3 | B3 | August 27 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 86.3 | B4 | August 27 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 40 | 226.9 | C1 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Input | +/- 40 | 91.6 | C2 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Input | +/- 40 | 143.8 | C3 | Sept 01 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Cell Death | +/- 40 | 122.8 | C4 | Sept 01 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Cell Death | +/- 40 | 119.8 | C5 | Sept 01 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Output | +/- 40 | 175.5 | C7 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Reset | +/- 40 | 174.2 | C8 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Reset | +/- 40 | 188.5 | C9 | Sept 01 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Filter | +/- 40 | 242.9 | D1 | Sept 01 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | pSB1A2 | Input: complete | +/- 40 | 193.5 | E7 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145278 K145278] | pSB1A2 | part for filter | +/- 42 | 185.2 | G4 | Sept 12 |
| [http://partsregistry.org/Part:BBa_K145275 K145275] | pSB1A2 | Filter | +/- 20 | 98.8 | H7 | Sept 16 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | pSB1A2 | Input: complete | +/- 40 | 200.7 | E8 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145253 K145253] | pSB1A2 | Inverter (old) - part 1 | +/- 40 | 223.3 | E9 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145253 K145253] | pSB1A2 | Inverter (old) - part 1 | +/- 40 | 202.9 | F1 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145258 K145258] | pSB1A2 | Memory - part 1 | +/- 40 | 209.4 | F7 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145279 K145279] | pSB1A2 | Input-Output (no tag) | +/- 40 | 333.0 | F8 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145280 K145280] | pSB1A2 | Input-Output (LVA) | +/- 40 | 251.0 | F9 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145251 K145251] | pSB1A2 | Reset (old): complete | +/- 40 | 168.6 | F2 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145251 K145251] | pSB1A2 | Reset (old): complete | +/- 40 | 154.5 | F3 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145205 K145205] | pSB1A2 | Output: complete | +/- 40 | 227.4 | F4 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145205 K145205] | pSB1A2 | Output: complete | +/- 40 | 215.2 | F5 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145254 K145254] | pSB1A2 | Ligation for Invertimer | +/- 40 | 137.6 | H3 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Filter | +/- 40 | 183.1 | D2 | Sept 01 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Inverter | +/- 40 | 56.0 | D3 | Sept 01 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Inverter | +/- 40 | 106.3 | D4 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 40 | 158.6 | B9 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Inverter | +/- 40 | 168.6 | G5 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 BE0240] | pSB1A2 | Control for K145150 promotor | +/- 40 | 178.4 | G6 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | pSB1A2 | Hybrid Promotor | +/- 40 | 97.6 | G7 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | pSB1A2 | Ligation for Memory | +/- 40 | 89 | G8 | Sept 09 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | Ligation for Cell Death | +/- 40 | 101.2 | H1 | Sept 14 |
| [http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Memory | +/- 30 | 141.3 | G9 | Sept 13 |
| [http://partsregistry.org/Part:BBa_K145280 K145280] | pSB1A2 | Input-Output (LVA) | +/- 30 | 275.9 | G2 | Sept 09 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353] | pSB1AK3 | Ligation for Memory | +/- 10 | 229.1 | H2 | Sept 09 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 150.2 | A6 | August 20 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 58.3 | A7 | August 20 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter (new key) | +/- 45 | 394.3 | G3 | Sept 12 |
| [http://partsregistry.org/Part:BBa_K145272 K145272] | pSB1A2 (Ap) | Ligation for Filter test | +/- 50 | 145.9 | G1 | Sept 13 |
| [http://partsregistry.org/Part:BBa_K145257 K145257] | pSB1A2 (Ap) | Ligation for Cell Death | +/- 50 | 55.1 | H4 | Sept 14 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:B0034 B0034] | pSB1A2 | Ligation for input | +/- 30 | 105,3 | H5 | Sept 16 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:B0034 B0034] | pSB1A2 | Ligation for input | +/- 30 | 113,4 | H6 | Sept 16 |
Digests (stored in the fridge in the lab at 8°C)
| Restriction Digests: parts |
|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_B0034 B0034] | EcoR I | Xba I | Vector | 2075 | 25 | - | 58 | September 11 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 11 | 11.7 | 1 | July 31 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | Pst I | Xba I | Insert | 144 | 25 | 3.8 | 3 | Sept 09 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 10 | 13.6 | 2 | August 26 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Xba I | Vector | 2092 | 5 | 17.6 | 25 | August 19 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Xba I | Vector | 2092 | 20 | 6.4 | 24 | August 29 |
| [http://partsregistry.org/Part:BBa_B0033 B0033] | EcoR I | Xba I | Vector | 2092 | 20 | 10.9 | 31 | August 27 |
| [http://partsregistry.org/Part:BBa_B0014 B0014] | EcoR I | Spe I | Insert | 110 | 20 | 1.3 | 50 | Sept 02 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | EcoR I | Spe I | Insert | 677 | 18 | 7.1 | 6 | July 24 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | Spe I | Pst I | Vector | 3192 | 25 | 16.4 | 32 | August 29 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | Spe I | Pst I | Vector | 2853 | 25 | 16.3 | 18 | August 29 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | Spe I | Pst I | Vector | 2724 | 25 | 19.2 | 7 | August 29 |
| [http://partsregistry.org/Part:BBa_E0040 E0040] | Spe I | Pst I | Vector | 2799 | 25 | 28.4 | 55 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145014 K145014] | Spe I | EcoR I | Insert | 2800 | 25 | 12.3 | 61 | Sept 14 |
| [http://partsregistry.org/Part:BBa_K145015 K145015] | Spe I | Pst I | Vector | 2823 | 25 | 18.8 | 33 | August 29 |
| [http://partsregistry.org/Part:BBa_K145151 K145151] | Spe I | Pst I | Vector | 2370 | 25 | 14.5 | 34 | August 29 |
| [http://partsregistry.org/Part:BBa_K145151 K145151] | Spe I | EcoR I | Vector | 2370 | 25 | 25.2 | 49 | August 29 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] | Spe I | Pst I | Vector | 4782 | 20 | 6.2 | 40 | August 29 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | Spe I | Pst I | Vector | 2680 | 20 | 17.5 | 39 | August 31 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | Spe I | Pst I | Vector | 2700 | 20 | 19.0 | 36 | August 31 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | EcoR I | Spe I | Insert | 803 | 28 | 5.4 | 17 | August 12 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | EcoR I | Spe I | Insert | 806 | 9 | 8.2 | 16 | August 01 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 11 | August 01 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 25 | 8.3 | 12 | August 27 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 28 | 5.0 | 10 | August 12 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | Pst I | Xba I | Insert | 771 | 40 | 5.3 | 56 | Sept 10 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | EcoR I | Xba I | Vector | 3956 | 26 | 4.5 | 30 | August 19 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 20 | July 30 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 21 | July 31 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
| [http://partsregistry.org/Part:BBa_J23100 J23100] | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 5 | July 24 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | Pst I | Spe I | Vector | 2220 | 21 | 8.6 | 47 | Sept 14 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 29 | July 29 |
| [http://partsregistry.org/Part:BBa_J23116 J23116] | EcoR I | Spe I | Insert | 52 | +/-20 | 8.1 | 26 | August 12 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] | EcoR I | Spe I | Insert | 2732 | +/-8 | 14.1 | 41 | August 12 |
| [http://partsregistry.org/Part:BBa_K145013 K145013] | EcoR I | Spe I | Insert | 660 | +/-25 | 20.8 | 53 | Sept 05 |
| [http://partsregistry.org/Part:BBa_K145150 K145150] | EcoR I | Spe I | Insert | 110 | +/-25 | 9.2 | 54 | Sept 05 |
| [http://partsregistry.org/Part:BBa_K145151 K145151] | Xba I | / | Vector | 2400 | 28 | - | 57 | August 12 |
| [http://partsregistry.org/Part:BBa_K145001 K145001] | EcoR I | Spe I | Insert | 2732 | +/-8 | 24.3 | 48 | August 12 |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] | EcoR I | Spe I | Plasmid | 2079 | 9 | 18.2 | 46 | August 01 |
| [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | EcoR I | Spe I | Plasmid | 2056 | 24 | 24.3 | 52 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 13 | July 30 |
| [http://partsregistry.org/Part:BBa_E0240 E0240] | EcoR I | Xba I | Vector | 2955 | 28 | 25.2 | 42 | Sept 01 |
| [http://partsregistry.org/Part:BBa_E0040 E0040] | EcoR I | Spe I | Insert | 720 | 28 | 8.3 | 37 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0056 C0056] | EcoR I | Spe I | Insert | 636 | 23 | 10.1 | 35 | Sept 01 |
| [http://partsregistry.org/Part:BBa_C0061 C0061] | EcoR I | Spe I | Insert | 618 | 28 | 9.0 | 38 | Sept 01 |
| [http://partsregistry.org/Part:BBa_P0152 P0152] | EcoR I | Xba I | Vector | 2930 | 28 | 23.2 | 43 | Sept 01 |
| [http://partsregistry.org/Part:BBa_P0353 P0353] | EcoR I | Xba I | Vector | 4000 | 28 | 15.3 | 45 | Sept 01 |
| [http://partsregistry.org/Part:BBa_J23066 J23066] | EcoR I | XbaI | Vector | 2700 | 28 | 11.4 | 27 | Sept 01 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 20 | 12.6 | 4 | Sept 01 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | EcoR I | Xba I | Vector | 2091 | 28 | 15.6 | 44 | Sept1 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 14 | July 30 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 25 | 2.1 | 15 | August 27 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | EcoR I | Spe I | Insert | 55 | 27 | 7.8 | 23 | August 07 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 8 | July 24 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 25 | 6.1 | 9 | July 29 |
| [http://partsregistry.org/Part:BBa_R0053 R0053] | EcoR I | Spe I | Insert | 70 | 20 | 1.1 | 51 | Sept 02 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | EcoR I | Spe I | Insert | 61 | 25 | 3.4 | 28 | July 29 |
| [http://partsregistry.org/Part:BBa_J23078 J23078] | Xba I | Pst I | Insert | 100 | 30 | 7.8 | 59 | Sept 13 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | Pst I | Spe I | Vector | 2820 | 30 | 8.0 | 60 | Sept 13 |
| Restriction Digests: Ligations |
|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
|---|---|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3350 | +/-30 | - | F3 | September 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4080 | +/-30 | 32.8 | A1 | August 14 |
| [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2800 | +/-30 | 17.3 | A2 | Sept 06 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066] | EcoR I | Spe I | Insert | 411 | +/-30 | 6.6 | A3 | Sept 06 |
| [http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152] | EcoR I | Spe I | Insert | 926 | +/-30 | 9.0 | A4 | Sept 06 |
| [http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033] | EcoR I | Spe I | Insert | 132 | +/-30 | 6.1 | A5 | Sept 06 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033] | EcoR I | Spe I | Insert | 86 | +/-30 | 4.1 | A6 | Sept 06 |
| [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3930 | +/-30 | 12.8 | A7 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4915 | +/-30 | 13.7 | A8 | Sept 06 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_B0034 B0034] | SpeI | Pst I | Vector | 2682 | +/-30 | 5.0 | D5 | Sept 10 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_B0034 B0034] | SpeI | Pst I | Vector | 2682 | +/-30 | 2.2 | E7 | Sept 14 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240] | EcoR I | Xba I | Vector | 2981 | +/-30 | 14.8 | A9 | Sept 06 |
| [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4086 | +/-30 | 14.8 | B1 | August 14 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 112 | +/-30 | 3.9 | B2 | August 14 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 101 | +/-30 | 4.0 | B3 | August 14 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0034 B0034] | EcoR I | Spe I | Insert | 68 | +/-30 | 3.0 | F4 | Sept 19 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | EcoR I | Spe I | Insert | 900 | +/-30 | 6.1 | F5 | Sept 19 |
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 4080 | +/-30 | 20.2 | F6 | Sept 19 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2986 | +/-25 | 17.8 | B4 | Sept 03 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2857 | +/-25 | 18.7 | B5 | Sept 03 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2956 | +/-25 | 20.8 | B6 | Sept 03 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2505 | +/-25 | 18.1 | B7 | Sept 03 |
| [http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | Pst I | Spe I | Vector | 2680 | +/-25 | 13.8 | F1 | Sept 15 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | EcoR I | Spe I | Insert | 900 | +/-10 | 7.3 | D1 | Sept 07 |
| [http://partsregistry.org/Part:BBa_K145257 K145257] | EcoR I | Xba I | Vector | 2642 | +/-20 | 18.7 | F2 | Sept 15 |
| [http://partsregistry.org/Part:BBa_K145272 K145272] | EcoR I | Xba I | Vector | 3039 | +/-20 | 18.5 | F3 | Sept 15 |
| [http://partsregistry.org/Part:BBa_K145201 K145201] | EcoR I | Xba I | Vector | 2900 | +/-20 | 25.2 | E8 | Sept 14 |
| [http://partsregistry.org/Part:BBa_K145205 K145205] | EcoR I | Xba I | Vector | 3065 | +/-20 | 26.9 | D2 | Sept 07 |
| [http://partsregistry.org/Part:BBa_K145253 K145253] | EcoR I | Spe I | Insert | 1410 | +/-20 | 10.9 | D3 | Sept 07 |
| [http://partsregistry.org/Part:BBa_K145251 K145251] | EcoR I | xba I | Vector | 3133 | +/-20 | 21.0 | D4 | Sept 07 |
| [http://partsregistry.org/Part:BBa_K145254 K145254] | EcoR I | Xba I | Vector | 2925 | +/-30 | - | D7 | Sept 10 |
| [http://partsregistry.org/Part:BBa_K145254 K145254] | EcoR I | Xba I | Vector | 2925 | +/-30 | 23.9 | E9 | Sept 14 |
| [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2505 | +/-30 | - | D9 | Sept 10 |
| [http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 E0240] | EcoR I | Xba I | Vector | 3004 | +/-30 | - | E1 | Sept 10 |
| [http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] | Spe I | Pst I | Vector | 2682 | +/-30 | - | E2 | Sept 10 |
| [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3327 | +/-25 | 18.1 | B8 | Sept 03 |
| [http://partsregistry.org/Part:BBa_E0040 E0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 2919 | +/-30 | - | D8 | Sept 10 |
| [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 71 | +/-28 | 2.5 | B9 | August 25 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-20 | 0.9 | C1 | August 22 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-30 | 2.9 | C2 | August 21 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 90 | +/-28 | 2.6 | C3 | August 25 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353] | EcoR I | Xba I | Vector | 4100 | +/-30 | - | D6 | Sept 10 |
| [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 90 | +/-25 | 3.8 | C4 | Sept 05 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] | EcoR I | Spe I | Insert | 311 | +/-28 | 3.6 | C5 | August 27 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] | EcoR I | Spe I | Insert | 144 | +/-28 | 2.4 | C6 | August 25 |
| [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022] | EcoR I | Spe I | Insert | 365 | +/-20 | 6.9 | C7 | August 14 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | Pst I | Spe I | Vector | 3468 | +/-30 | 7.9 | E5 | Sept 13 |
| [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Spe I | Insert | 500 | +/-30 | 6.9 | E6 | Sept 13 |
Things stored in freezer in lab (-20°C)
| Miscellaneous |
|---|
| Description | Name | µl left | Concentration | Box | Date |
|---|---|---|---|---|---|
| Primer GFP-LVA | 1084 | 90 | 20 µM | 2 | August 01 |
| Primer GFP-LVA | 1085 | 90 | 20 µM | 4 | August 01 |
| dNTP mix | ATP, GTP, TTP, CTP | 200 | 10 mM | 81 | August 28 |
| Primer GFP-LVA | 1084 | 500 | 100 µM | 1 | August 01 |
| Primer GFP-LVA | 1085 | 500 | 100 µM | 3 | August 01 |
| Primer Test | 1074 | 800 | 100 µM | 5 | August 07 |
| Primer Test | 1075 | 780 | 100 µM | 7 | August 07 |
| Primer T7-UmuD | 1076 | 560 | 100 µM | 9 | August 07 |
| Primer T7-UmuD | 1077 | 477 | 100 µM | 11 | August 07 |
| Primer T7-UmuD | 1078 | 467 | 100 µM | 13 | August 07 |
| Primer T7-UmuD | 1079 | 45 | 100 µM | 15 | August 07 |
| Primer Transduction | 1097 | 627 | 100 µM | 17 | August 07 |
| Primer Transduction | 1098 | 710 | 100 µM | 19 | August 07 |
| Primer Transduction | 1099 | 608 | 100 µM | 21 | August 07 |
| Primer Anti-sense LuxI | 1100 | 522 | 100 µM | 23 | August 07 |
| Primer Anti-sense LuxI | 1101 | 237 | 100 µM | 25 | August 07 |
| Primer Test | 1074 | 100 | 20 µM | 6 | August 07 |
| Primer Test | 1075 | 100 | 20 µM | 8 | August 07 |
| Primer T7-UmuD | 1076 | 100 | 20 µM | 10 | August 07 |
| Primer T7-UmuD | 1077 | 100 | 20 µM | 12 | August 07 |
| Primer T7-UmuD | 1078 | 100 | 20 µM | 14 | August 07 |
| Primer T7-UmuD | 1079 | 100 | 20 µM | 16 | August 07 |
| Primer Transduction | 1097 | 100 | 20 µM | 18 | August 07 |
| Primer Transduction | 1098 | 100 | 20 µM | 20 | August 07 |
| Primer Transduction | 1099 | 100 | 20 µM | 22 | August 07 |
| Primer Anti-sense LuxI | 1100 | 100 | 20 µM | 24 | August 07 |
| Primer Anti-sense LuxI | 1101 | 100 | 20 µM | 26 | August 07 |
| dNTP mix | ATP, GTP, TTP, CTP | 90 | 2 µM | 80 | August 19 |
| Primer ccdB | 1127 | 100 | 100 µM | 27 | August 19 |
| Primer ccdB | 1128 | 100 | 100 µM | 29 | August 19 |
| Primer K145150 | 1129 | 100 | 100 µM | 31 | August 19 |
| Primer K145150 | 1130 | 100 | 100 µM | 34 | August 19 |
| Primer ccdB | 1127 | 90 | 20 µM | 28 | August 19 |
| Primer ccdB | 1128 | 980 | 20 µM | 30 | August 19 |
| Primer K145150 | 1129 | 990 | 1 µM | 33 | August 19 |
| Primer K145150 | 1129 | 90 | 20 µM | 32 | August 19 |
| Primer K145150 | 1130 | 990 | 1 µM | 36 | August 19 |
| Primer K145150 | 1130 | 90 | 20 µM | 35 | August 19 |
| Primer K145014 (2.1) | 1156 | 990 | 100 µM | 37 | Sept 01 |
| Primer K145014 (2.1) | 1156 | 90 | 20 µM | 38 | Sept 01 |
| Primer Suffix | 1153 | 990 | 100 µM | 39 | Sept 02 |
| Primer Suffix | 1153 | 90 | 20 µM | 40 | Sept 02 |
| Primer Prefix | 1152 | 990 | 100 µM | 41 | Sept 02 |
| Primer Prefix | 1152 | 90 | 20 µM | 42 | Sept 02 |
