Team:Hawaii/Notebook/2008-09-26
From 2008.igem.org
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===Verification of Transformants=== | ===Verification of Transformants=== | ||
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
- | [[Image:092608colonyPCR.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. | + | [[Image:092608colonyPCR.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1.5 hours. Two microliters of PCR product were loaded into each well.]] |
{|class=wikitable border=1 align=center | {|class=wikitable border=1 align=center | ||
!Construct | !Construct |
Revision as of 00:00, 27 September 2008
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of Transformants
- Grace
Construct | Colonies |
---|---|
nir+rbs+GFP + tt | 3 |
nir + rbs+GFP+tt1 | 1 |
plac + rbs+GFP+tt1 | 1 |
nir + rbs+GFP+tt2 | 5 |
plac + rbs+GFP+tt2 | 0 |
pSB1A3 to self (- control) | 0 |
J33207 + pRL1383a (plated on IPTG+Xgal) | |
J33207 + pRL1383a (1:10) (plated on Xgal) | |
pRL1383a to self (+ control) | |
J33207 plasmid (+ control, plated on IPTG+Xgal) | 162 (all blue!) |
pRL1383a plasmid (+ control) | |
no DNA | 0 |
- Colony PCR of transformants
Drylab Work
iGEM presentation
- Margaret, Krystle, Grace
- Reviewed Margaret's presentation for Dr. Lee and outlined presentation for Boston.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]