Team:Chiba/jk/β/week 5

From 2008.igem.org

(Difference between revisions)
(19 September, 2008)
(17 September, 2008)
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**conditions
**conditions
#strain<br>Senders:JW1908<br>Receiver:JW1908<br>
#strain<br>Senders:JW1908<br>Receiver:JW1908<br>
 +
#temparature<br>37℃ and 30℃
 +
 +
*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
 +
**conditions
 +
#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br>
#temparature<br>37℃ and 30℃
#temparature<br>37℃ and 30℃

Revision as of 11:13, 30 September 2008

>送受信班

>Protocols

Contents

Week 5

>last week

14 September, 2008

(13/9)--->Mini prep


Transformation
Competent cells : XL10G
  • BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
  • Plac+RhlI+LVA
  • Plac+CinI+LVA
--->(15/9)

15 September, 2008

Transformation
competent cells : JW1908
  • BBa_S03623 [http://partsregistry.org/Part:BBa_S03623]
  • BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]
  • BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]
  • BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]
  • BBa_T9002 [http://partsregistry.org/Part:BBa_T9002]

(15/9)-->T9002 check(AHL sensitivity and time response test)

16 September, 2008

(15/9)->

  1. strain
    Senders:JW1908
    Receiver:JW1908
  2. temparature
    37℃ and 30℃

17 September, 2008

  1. strain
    Senders:JW1908
    Receiver:JW1908
  2. temparature
    37℃ and 30℃
  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃

18 September, 2008

  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃

19 September, 2008

  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    30℃ and Room Temparature

20 September, 2008

>next week

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