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Team:Chiba/jk/β/week 5
From 2008.igem.org
>送受信班
Contents |
Week 5
>last week
14 September, 2008
- (13/9)--->Mini prep
- Transformation
- Competent cells : XL10G
- BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
- Plac+RhlI+LVA
- Plac+CinI+LVA
- --->(15/9)
15 September, 2008
- Transformation
- competent cells : JW1908
- BBa_S03623 [http://partsregistry.org/Part:BBa_S03623]
- BBa_K084007 [http://partsregistry.org/Part:BBa_K084007]
- BBa_K084008 [http://partsregistry.org/Part:BBa_K084008]
- BBa_K084009 [http://partsregistry.org/Part:BBa_K084009]
- BBa_T9002 [http://partsregistry.org/Part:BBa_T9002]
(15/9)-->T9002 check(AHL sensitivity and time response test)
16 September, 2008
(15/9)->
- time-delay test
- conditions
- strain
Senders:JW1908
Receiver:JW1908
- temparature
37℃ and 30℃
17 September, 2008
- time-delay test
- conditions
- strain
Senders:JW1908
Receiver:JW1908
- temparature
37℃ and 30℃
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37℃ and 30℃
18 September, 2008
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37℃ and 30℃
19 September, 2008
-->(?/9) Gel Check
| Sample Name | J04500 | R0010 | C0161 | Cin |
| Sample DNA | 30 | 30 | 90 | ? |
| Loading Dye | 6 | 6 | 18 | ? |
| TOTAL | 36 | 36 | 108 | 24 |
--->Gel Extract
(各サンプル well３つずつ)
- note:ADB buffer添加後,37℃,6h
- --->Zymo Clean
- elution
- J0450-->27
- C0161-->11μL
--->Gel Check
- --->Ligation
| Sample Name | insert+vector | insert | vector |
| C0161 | 3 | 3 | - |
| J04500 | 4.5 | - | 4.5 | ligase | 1 | 1 | 1 |
| Buffer(invitrogen) | 3 | 2 | 2 |
| NFW? | 3.5 | 4 | 2.5 |
| TOTAL | 15 | 10 | 10 |
- --->Transformation above ligation onto a LB-amp plate.
- note
- competent cell:XL10GOLD
- DNA:1μL,SOC:120μL
--->(20/9)Mini prep
- time-delay test
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
30℃ and Room Temparature
20 September, 2008
--->(19/9)Mini prep
--->Gel Check
| Sample Name | Lagation(J04500+C0161) |
| Sample DNA | 1 |
| Loading Dye | 1 |
| H2O | 4 |
| TOTAL | 6 |
- From left;
- -> OK
- -> OK
- -> OK
- -> OK
- --->Digestion Test
| Sample No. | 1-4 | 5-8 |
| Sample DNA | 3 | 3 |
| XbaⅠ | 0.25? | 0.25 |
| SpeⅠ | 0.5? | 0.5 |
| Buffer 2 | 2 | 2 |
| BSA | 2? | 2? |
| dH2O | ? | 12.5 |
| TOTAL | 20 | 20 |
- --->Gel Check
Sample No. 1-4 5~8 Sample DNA 20 20 Loading Dye 4 4 TOTAL 24 24 - From left;
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
- -> OK
--->(?/9)Transformation
--->(22/9)phenotype check
>next week
ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート - From left;
