Alberta NINT/2 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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Ligated K102002 into pSB4A5 and K102008 into pSB4A5 (using pSB4A5 as a negative control). | Ligated K102002 into pSB4A5 and K102008 into pSB4A5 (using pSB4A5 as a negative control). | ||
Transformed XL1-B cells with these ligations. | Transformed XL1-B cells with these ligations. | ||
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Latest revision as of 22:55, 11 October 2008
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lab work (SD):
Extracted DNA from gel fragments (~600 bp) for K102002 and K102008 digested with EcoRI + PstI. Ligated K102002 into pSB4A5 and K102008 into pSB4A5 (using pSB4A5 as a negative control). Transformed XL1-B cells with these ligations.