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Team:Waterloo
From 2008.igem.org
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Our goal is to engineer a genome-free, cell-based expression system capable of producing a desired protein in response to environmental signals. The genome will be degraded by the combined activity of a restriction endonuclease (to fragment the genome) and an exonuclease (to hasten degradation of the genome). The gene for the protein of interest will be located on a plasmid which will lack recognition sites for the endonuclease, enabling it to remain intact after genome degradation. Expression of plasmid genes is expected to continue for a period of time until the "cell" expires. | Our goal is to engineer a genome-free, cell-based expression system capable of producing a desired protein in response to environmental signals. The genome will be degraded by the combined activity of a restriction endonuclease (to fragment the genome) and an exonuclease (to hasten degradation of the genome). The gene for the protein of interest will be located on a plasmid which will lack recognition sites for the endonuclease, enabling it to remain intact after genome degradation. Expression of plasmid genes is expected to continue for a period of time until the "cell" expires. | ||
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Revision as of 03:05, 15 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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| The UW iGEM team is an interdisciplinary undergraduate-driven group spanning the faculties of Science, Mathematics, and Engineering. Our undergraduate members and graduate and faculty advisors bring skills, expertise, and perspectives from a broad range of fields, including Biology, Biochemistry, Computer Science, Bioinformatics, Computer and Electrical Engineering, Chemical Engineering, and Mathematical Physics. |  |
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Our goal is to engineer a genome-free, cell-based expression system capable of producing a desired protein in response to environmental signals. The genome will be degraded by the combined activity of a restriction endonuclease (to fragment the genome) and an exonuclease (to hasten degradation of the genome). The gene for the protein of interest will be located on a plasmid which will lack recognition sites for the endonuclease, enabling it to remain intact after genome degradation. Expression of plasmid genes is expected to continue for a period of time until the "cell" expires. | 
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