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Team:Hawaii/Notebook/2008-10-20
From 2008.igem.org
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(→Drylab Work) |
(→PCR of J04430 (lac+rbs+GFP+tt+tt)) |
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:* Will act as (-) control for GFP secretion in cyanos | :* Will act as (-) control for GFP secretion in cyanos | ||
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| + | ===Overnight RE digest=== | ||
| + | :<strong> Grace</strong> | ||
| + | |||
| + | :* EcoRI and PstI digest of BBpRL1383a and J04430 | ||
| + | |||
| + | ===Made LB+sp+sm plates for antibiotic gradient test on Wednesday=== | ||
| + | :<strong>Grace <strong> | ||
| + | |||
| + | :* 30 ml plates | ||
= Discussion = | = Discussion = | ||
Revision as of 00:00, 21 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Large scale plasmid prep of BB-pRL1383a (blue)
- Grace
PCR of J04430 (lac+rbs+GFP+tt+tt)
- Grace
- Will act as (-) control for GFP secretion in cyanos
Overnight RE digest
- Grace
- EcoRI and PstI digest of BBpRL1383a and J04430
Made LB+sp+sm plates for antibiotic gradient test on Wednesday
- Grace <strong>
- 30 ml plates
Discussion
Quote of the Day
Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
