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Team:Hawaii/Notebook/2008-10-21
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Insertion of lac-GFP device into BB-pRL1383a=== :<strong> Grace</strong> [[Image:102108REdigests.png|right|thumb|EtBr ...) |
(→Antibiotic test for BB-pRL1383a) |
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:* Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a [[ng]] | :* Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a [[ng]] | ||
:* Plated 50 &m;ul cells + SOB on each plate | :* Plated 50 &m;ul cells + SOB on each plate | ||
| + | :* Incubated at 37C for 2 days | ||
= Discussion = | = Discussion = | ||
Revision as of 18:51, 21 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Insertion of lac-GFP device into BB-pRL1383a
- Grace
- Ran RE digests on gel
- Extracted bands from gel
- Ligated J04430 and BB-pRL1383a
- Used 5μl to transform DH5α
Antibiotic test for BB-pRL1383a
- Grace
- Made LB+spvariable plates with 40 μl 20X X-gal
- Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a ng
- Plated 50 &m;ul cells + SOB on each plate
- Incubated at 37C for 2 days
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
