Team:Hawaii/Notebook/2008-10-21

From 2008.igem.org

(Difference between revisions)
(Antibiotic test for BB-pRL1383a)
(Antibiotic test for BB-pRL1383a)
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:* Made LB+sp<sub>variable</sub> plates with 40 &mu;l 20X X-gal
:* Made LB+sp<sub>variable</sub> plates with 40 &mu;l 20X X-gal
-
:* Transformed 100 &mu;l DH5&alpha; with 2 &mul; BB-pRL1383a [[ng]]
+
:* Transformed 100 &mu;l DH5&alpha; with 2 &mul; BB-pRL1383a (4 ng)
:* Plated 50 &m;ul cells + SOB on each plate
:* Plated 50 &m;ul cells + SOB on each plate
:* Incubated at 37C for 2 days
:* Incubated at 37C for 2 days

Revision as of 21:26, 21 October 2008

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Notebook (t) Meetings (t)

Things we did today

Wetlab work

Insertion of lac-GFP device into BB-pRL1383a

Grace
EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.
  • Ran RE digests on gel
  • Extracted bands from gel
  • Ligated J04430 and BB-pRL1383a
  • Used 5μl to transform DH5α

Antibiotic test for BB-pRL1383a

Grace
  • Made LB+spvariable plates with 40 μl 20X X-gal
  • Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a (4 ng)
  • Plated 50 &m;ul cells + SOB on each plate
  • Incubated at 37C for 2 days

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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