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Team:Hawaii/Notebook/2008-10-21
From 2008.igem.org
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(→Antibiotic test for BB-pRL1383a) |
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[[Image:102108REdigests.png|right|thumb|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]] | [[Image:102108REdigests.png|right|thumb|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]] | ||
:*Ran RE digests on gel | :*Ran RE digests on gel | ||
| - | ::* | + | ::*Bands were all incorrect size |
| - | :* | + | :* Reinoculated TB+sp<sub>100</sub> for plasmid prep tomorrow |
| - | :* | + | :* PCR of J04430 from filter paper to verify part; used H/B primers |
| - | + | ||
===Antibiotic test for BB-pRL1383a=== | ===Antibiotic test for BB-pRL1383a=== | ||
Revision as of 21:27, 21 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Insertion of lac-GFP device into BB-pRL1383a
- Grace
- Ran RE digests on gel
- Bands were all incorrect size
- Reinoculated TB+sp100 for plasmid prep tomorrow
- PCR of J04430 from filter paper to verify part; used H/B primers
Antibiotic test for BB-pRL1383a
- Grace
- Made LB+spvariable plates with 40 μl 20X X-gal
- Transformed 100 μl DH5α with 2 &mul; BB-pRL1383a (4 ng)
- Plated 50 &m;ul cells + SOB on each plate
- Incubated at 37C for 2 days
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
