Team:Hawaii/Notebook/2008-10-22

From 2008.igem.org

(Difference between revisions)
Gracek (Talk | contribs)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid prep of BB-pRL1383a=== :<strong> Grace</strong> :* ''E. coli'' did not grow. Reinoculated. ===Extracted J0443...)
Newer edit →

Revision as of 20:14, 22 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Plasmid prep of BB-pRL1383a

Grace
  • E. coli did not grow. Reinoculated.

Extracted J04430 from filter paper

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Ten microliters of PCR product were loaded.
  • Used 2 μl of filter paper solution for PCR to verify plasmid

Transformation

Grace
  • Used 2 μl each of BBpRL1383a-1 and J04430 (from filter) to transform DB3.1


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]