Revision as of 08:56, 23 October 2008

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Things we did today

Grace

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.

Grace

Made LB+sp_variable plates with 50 μl 20X X-gal
Transformed 100 μl DH5α with 2 μl BB-pRL1383a (4 ng)
Plated 50 μl cells + SOB on each LB+sp plate; plated 20 μl untransformed cells + SOB on each control plate
Incubated at 37C for 2 days

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Insertion of lac-GFP device into BB-pRL1383a===
 :<strong>  Grace</strong>
-[[Image:102108REdigests.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]][[Image:102108J04430.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.]]
 :*Ran RE digests on gel
 ::*Bands were all incorrect size
 :* Reinoculated TB+sp<sub>100</sub> for plasmid prep tomorrow
 :* PCR of J04430 from filter paper to verify part; used H/B primers
+[[Image:102108REdigests.png|left|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]][[Image:102108J04430.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.]]
 ===[[Team:Hawaii/Antibiotic test for BB-pRL1383a]]===