Team:Hawaii/Notebook/2008-10-21

From 2008.igem.org

(Difference between revisions)
(Insertion of lac-GFP device into BB-pRL1383a)
(Insertion of lac-GFP device into BB-pRL1383a)
Line 4: Line 4:
== Wetlab work ==
== Wetlab work ==
===Insertion of lac-GFP device into BB-pRL1383a===
===Insertion of lac-GFP device into BB-pRL1383a===
 +
[[Image:102108REdigests.png|right|thumb|150px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]][[Image:102108J04430.png|right|thumb|150px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.]]
 +
:<strong>  Grace</strong>
:<strong>  Grace</strong>
Line 10: Line 12:
:* Reinoculated TB+sp<sub>100</sub> for plasmid prep tomorrow
:* Reinoculated TB+sp<sub>100</sub> for plasmid prep tomorrow
:* PCR of J04430 from filter paper to verify part; used H/B primers
:* PCR of J04430 from filter paper to verify part; used H/B primers
-
[[Image:102108REdigests.png|left|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.]][[Image:102108J04430.png|right|thumb|200px|EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.]]
 
===[[Team:Hawaii/Antibiotic test for BB-pRL1383a]]===
===[[Team:Hawaii/Antibiotic test for BB-pRL1383a]]===

Revision as of 08:56, 23 October 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Insertion of lac-GFP device into BB-pRL1383a

EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Thirty microliters of the RE digest reaction were loaded into each well.
EtBr stained 0.8% agarose gel ran at 60V for 90 minutes. Ten microliters of PCR product were loaded.
Grace
  • Ran RE digests on gel
  • Bands were all incorrect size
  • Reinoculated TB+sp100 for plasmid prep tomorrow
  • PCR of J04430 from filter paper to verify part; used H/B primers

Team:Hawaii/Antibiotic test for BB-pRL1383a

Grace
  • Made LB+spvariable plates with 50 μl 20X X-gal
  • Transformed 100 μl DH5α with 2 μl BB-pRL1383a (4 ng)
  • Plated 50 μl cells + SOB on each LB+sp plate; plated 20 μl untransformed cells + SOB on each control plate
  • Incubated at 37C for 2 days

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]