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Team:Hawaii/Notebook/2008-06-13
From 2008.igem.org
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:* Made BG-11 plates for retest next week. | :* Made BG-11 plates for retest next week. | ||
| - | === | + | ===Made LB agar stock=== |
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
| - | + | :* (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C. | |
| - | :* (2) 300 mL batches of LB + 1.5% Agar | + | |
== Drylab Work == | == Drylab Work == | ||
Revision as of 02:08, 14 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Prepped Cells for Glycerol Stock/Seed Stock
- Krystle
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. worked on <blah experiment link>, PCR, ran gel
Prepped Cells for Competency
- Grace
- Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
- Made SOB for new inoculation.
- Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).
Cell density test
- Grace
- Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
- Made BG-11 plates for retest next week.
Made LB agar stock
- Grace
- (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
- We need to make more BG-ll 50x stock solution and TES 50x stock solution
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
