Team:Hawaii/Notebook/2008-06-13

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===Prepped Cells for Glycerol Stock/Seed Stock===
===Prepped Cells for Glycerol Stock/Seed Stock===
:<strong> Krystle</strong>
:<strong> Krystle</strong>
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Inoculated a single colony in each 10 mL LB + antibiotic<br>
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
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<br>
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:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
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<table style="text-align: left; width: 478px; height: 205px;"
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border="1" cellpadding="2" cellspacing="2">
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  <tbody>
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    <tr>
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      <td style="font-weight: bold; text-align: center;">Antibiotic</td>
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      <td style="font-weight: bold; text-align: center;">Cell
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Type</td>
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      <td style="font-weight: bold; text-align: center;">Plasmid</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;" colspan="1"
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rowspan="4">Amp<small><small>100</small></small></td>
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      <td style="text-align: center;">supercompetent cells</td>
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      <td style="text-align: center;">pUC18</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;">DH5alpha</td>
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      <td style="text-align: center;">GFP</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;">DH5alpha</td>
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      <td style="text-align: center;">tet+GFP</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;">DH5alpha</td>
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      <td style="text-align: center;">RBS</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;">Kan<small><small>50</small></small></td>
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      <td style="text-align: center;">E.coli</td>
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      <td style="text-align: center;">RK1</td>
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    </tr>
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    <tr>
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      <td style="text-align: center;">Sm<small><small>2.5</small></small>
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+ Sp <small><small>2.5</small></small></td>
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      <td style="text-align: center;">DH5alpha</td>
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      <td style="text-align: center;">pRL1383a</td>
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    </tr>
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  </tbody>
===Prepped Cells for Competency===
===Prepped Cells for Competency===

Revision as of 02:11, 14 June 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Prepped Cells for Glycerol Stock/Seed Stock

Krystle

Inoculated a single colony in each 10 mL LB + antibiotic

<tbody> </tbody>

Prepped Cells for Competency

Grace
  • Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
  • Made SOB for new inoculation.
  • Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).

Cell density test

Grace
  • Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
  • Made BG-11 plates for retest next week.

Made LB agar stock

Grace
  • (2) 300 mL batches of LB + 1.5% Agar. Stored in 4C.

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

  • We need to make more BG-ll 50x stock solution and TES 50x stock solution
  • Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
  • Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


Antibiotic Cell Type Plasmid
Amp100 supercompetent cells pUC18
DH5alpha GFP
DH5alpha tet+GFP
DH5alpha RBS
Kan50 E.coli RK1
Sm2.5 + Sp 2.5 DH5alpha pRL1383a
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