Revision as of 06:09, 14 June 2008

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Things we did today

Krystle

Inoculated a single colony in each 10 mL LB + antibiotic

Grace

Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
Made SOB for new inoculation.
Inoculated 250 μl SOB with 100 μl DH5α. Inoculated 250 μl SOB with 10 μl DH5α. Incubated overnight at 27C with shaking (150 rpm).

Grace

Retrieved plates from SC incubator. All plates had too many colonies (lawn). Need to redo.
Made BG-11 plates for retest next week.

Grace

We need to make more BG-ll 50x stock solution and TES 50x stock solution
Kelly from SC lab will be in 12pm-3pm. We should retrieve our triparental conjugation plates then.
Grace, Krystle, and Norman will come in at 9am tomorrow to plate triparental conjugates, make competent cells, and glycerol stock purified Biobrick colonies.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

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@@ Line 52: / Line 52: @@
 :* Checked yesterday's inoculation. OD600=1.2 (too high). Need to redo.
 :* Made SOB for new inoculation.
-:* Inoculated 250 &mu;l SOB with 100 &mu;l DH5&alpha;. Inoculated 250 &mu;l SOB with 10 &mu;l DH5&alpha. Incubated overnight at 27C with shaking (150 rpm).
+:* Inoculated 250 &mu;l SOB with 100 &mu;l DH5&alpha;. Inoculated 250 &mu;l SOB with 10 &mu;l DH5&alpha;. Incubated overnight at 27C with shaking (150 rpm).
 === Cell density test===
@@ Line 66: / Line 66: @@
 == Drylab Work ==
-===Name of Task===
-:<strong> name of person/people who performed the task</strong>
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
-:* e.g. read through papers, worked on proposal, etc.