Team:Chiba/Calendar-Home/19 August 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 06:50, 24 October 2008

>Home | Notebook

18 August 2008 <|> 20 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

PCR
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control


DNA Template 1
dNTP mix 10
Foward Primer 5
Reverse Primer 5
DNA polymerase VENT 1
Thermopol Buffer 5
dH2O 28
TOTAL 50μL


95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )・・・25cycles -> 72℃,10min -> 6℃


--->Gel Check
Chiba-0819.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control ->OK


Team:Output

no work