Team:Chiba/Calendar-Home/9 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
Line 7: Line 7:
===Team:Communication===
===Team:Communication===
 +
(6/9)--->PCR of I9030
-
-->(10/9)
+
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-
:Colony Count
+
-
#LuxI
+
-
#LuxI with LVA
+
-
#Las
+
-
#Background(R0010)
+
-
#Background(C0261)
+
-
Pick colony
+
--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
-
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
+
--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
-
::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
+
-
 
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">DNA Template</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP mix</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Foward Primer</td>
+
-
<td>0.3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Reverse Primer</td>
+
-
<td>0.3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>taq DNA polymerase</td>
+
-
<td>0.5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermopol Buffer</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Nuclease free water</td>
+
-
<td>20</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>30μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
??
+
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+
-
??
+
-
 
+
-
-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+
-
 
+
-
--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]'''
+
-
 
+
-
elute 50μL
+

Revision as of 07:42, 24 October 2008

>Home | Notebook

8 September 2008 <|> 10 September 2008

Laboratory work

Team:Input

no work

Team:Communication

(6/9)--->PCR of I9030

--->Gel Check

--->Gel Extract

--->Zymo Clean

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/9_September_2008"