Team:Warsaw/Calendar-Main/31 August 2008
From 2008.igem.org
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- | < | + | <h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3> |
+ | <h4>Michał L., Ewa</h4> | ||
+ | <ol> | ||
+ | <li>0.5 ml of each liquid culture was plated on solid LB supplemented with 200 ug/ml rifampicin + Tetracycline</li> | ||
+ | <li>0.1 ml of each liquid culture was plated on solid CTAXI medium</li> | ||
+ | <li>Plasmids were isolated from the rest of cultures.</li> | ||
+ | <li>Gel electrophoresis of iolated plasmids and gel-out of pZC320</li> | ||
+ | <li>Gel-outed plasmids were transformed to Top10 cells</li> | ||
+ | </ol> | ||
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Revision as of 16:36, 24 October 2008
Blue-white screening and rifampicin test in GM2163Michał L., Ewa
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