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Team:UC Berkeley/GatewayPlasmid
From 2008.igem.org
(Difference between revisions)
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=''' Gateway Plasmid Schemes '''= | =''' Gateway Plasmid Schemes '''= | ||
| - | == '''Scheme 1: Gateway | + | == '''Scheme 1: Gateway Machinery in Assembly Vector '''== |
Two different variations of pBjh1601CA assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below). <br> | Two different variations of pBjh1601CA assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below). <br> | ||
| - | + | <br> | |
[[Image:pK112245.png]] <br> | [[Image:pK112245.png]] <br> | ||
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The entry vector used in both cases was pBca1256 (see image below). | The entry vector used in both cases was pBca1256 (see image below). | ||
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[[Image:pBca 1256.png]] <br> | [[Image:pBca 1256.png]] <br> | ||
| + | |||
| + | == '''Scheme 2: Gateway Machinery in Entry Vector '''== | ||
| + | |||
[[Image:pK112247.png]] <br> | [[Image:pK112247.png]] <br> | ||
[[Image:pK112248.png]] <br> | [[Image:pK112248.png]] <br> | ||
[[Image:pK112128.png]] <br> | [[Image:pK112128.png]] <br> | ||
Revision as of 04:06, 28 October 2008
Gateway Plasmid Schemes
Scheme 1: Gateway Machinery in Assembly Vector
Two different variations of pBjh1601CA assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below).
The entry vector used in both cases was pBca1256 (see image below).
Scheme 2: Gateway Machinery in Entry Vector
