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Team:UC Berkeley/GatewayPlasmid
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== '''General Procedures''' == | == '''General Procedures''' == | ||
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Revision as of 05:42, 28 October 2008
Contents |
Gateway Plasmid Schemes
Scheme 1: Gateway Machinery in Assembly Vector
Assembly Vectors:Two different variations of the pK112128 assembly vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} between the ccdB site and the attR2 site. These plasmids are named pK112245 and pK112246 respectively (see images below).
Entry Vector:The entry vector used in both cases was pBca1256 (see image below).
Scheme 2: Gateway Machinery in Entry Vector
Assembly Vector:The assembly vector used for each of the following entry vector variations is pK112128 (see image below.
Entry Vectors: Two different variations of the pBca1256 entry vector were created: one with {promoter.xis.int!} and one with {promoter.xis.int!}{rbs.ihfA!}{rbs.ihfB!} just before the attL1 site. These plasmids are named pK112247 and pK112248 respectively (see images below).
