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Making Overnight Cultures from Agar Colonies
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* Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | * Pipette 10mL LB into red-capped culture tubes or conical shake flasks. | ||
* Using an autoclaved toothpick, gently touch the point to a single colony. | * Using an autoclaved toothpick, gently touch the point to a single colony. | ||
* Drop the toothpick point down into the LB. | * Drop the toothpick point down into the LB. | ||
* Incubate overnight at 37°C whilst shaking. | * Incubate overnight at 37°C whilst shaking. | ||
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Latest revision as of 16:08, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
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Home >> Wet Lab >> Protocols >> Making Overnight Cultures from Agar Colonies
- Pipette 10mL LB into red-capped culture tubes or conical shake flasks.
- Using an autoclaved toothpick, gently touch the point to a single colony.
- Drop the toothpick point down into the LB.
- Incubate overnight at 37°C whilst shaking.
