"
Team:Waterloo/Notebook/Protocols/Glycerol Stock Preparation
From 2008.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
| + | !align="center"|[[Team:Waterloo|Home]] | ||
| + | !align="center"|[[Team:Waterloo/Team|The Team]] | ||
| + | !align="center"|[[Team:Waterloo/Project|The Project]] | ||
| + | !align="center"|[[Team:Waterloo/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Waterloo/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Waterloo/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Waterloo/Sponsors|Sponsors]] | ||
| + | |} | ||
| + | |||
<b><u><big>Glycerol Stock Preparation </big></u></b><br> | <b><u><big>Glycerol Stock Preparation </big></u></b><br> | ||
Latest revision as of 22:54, 28 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook | Sponsors |
|---|
Glycerol Stock Preparation
Before you start
- It is important to use aseptic technique when carrying out the procedures
Materials
5 mL culture tube
60% Glycerol
Sterile screw-top cryotube
Instructions
1. Inoculate a 5 mL culture tube.
2. Incubate overnight on the shaker.
3. Pipette 500 μL of 60% glycerol culture into a sterile screw-top cryotube.
4. Piptette 500 μL of culture into the cryotube.
5. Label and date cryotube.
6. Vortex.
7. Cover the label with clear tape.
8. Store in -80°C freezer.
9. Add strain to database.
