Team:Chiba/Calendar-Home/19 August 2008

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:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
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:95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

Revision as of 18:15, 29 October 2008

>Home | Notebook

18 August 2008 <|> 20 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

PCR
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control


DNA Template 1
dNTP mix 10
Foward Primer 5
Reverse Primer 5
DNA polymerase VENT 1
Thermopol Buffer 5
dH2O 28
TOTAL 50μL


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


--->Gel Check
Chiba-0819.JPG
Sample DNA 3
Loading Dye 2
dH2O 7
TOTAL 12μL
From left;
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) ->None
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) ->None
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) ->None
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) ->None
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2007) ->None
  • [http://partsregistry.org/Part:BBa_S3154 BBa_S3154](2006) ->None
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK

Team:Output

no work

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