Team:Chiba/Calendar-Home/8 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Output)
(→Team:Output)
Line 18: Line 18:
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
-
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
+
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①</td><td>⑤</td>
+
<td>1</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
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-->left at 65 degrees for 30 minutes.
-->left at 65 degrees for 30 minutes.
-
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](①:21μl,⑤:6μl)
+
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](1:21μl,5:6μl)
Line 76: Line 76:
</table>
</table>
-
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl  
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl  
-
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160]④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
-
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
<td width="257">Sample No.</td>
<td width="257">Sample No.</td>
-
<td>①&②</td><td>①&③</td><td>①&④</td><td>①</td><td>⑤&⑥</td><td>⑤</td>
+
<td>1&2</td><td>1&3</td><td>1&4</td><td>1</td><td>5&6</td><td>5</td>
</tr>
</tr>
<tr>
<tr>

Revision as of 22:10, 29 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract


--->Zymo Clean
eluted with 15μL(?) of NFW.

Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5)
Sample No. 15
DNA(digested sample)(μL) 76
SAP(μL) 22
SAP Buffer(μL) 33
TOTAL(μL) 3030

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(1:21μl,5:6μl)


-->Gel check

Sample No. each
DNA(digested sample)(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1) 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 BBa_S03160](4) 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
Sample No. 1&21&31&415&65
DNA(vector)(μL) 333352.1
DNA(insert)(μL) 333-3.1-
Ligase(μL) 111111
Ligase Buffer(x5)(μL) 222222
dH2O(μL) 11148.94
TOTAL(μL) 101010102010
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