Team:Chiba/Calendar-Home/7 September 2008

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6 September 2008 <|> 8 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : BBa_R0010(unknown)

Sample DNA 2 : BBa_R0010(100ng/μL)

Sample No.	1,2
Sample DNA(μL)	12
PstI(μL)	0.5
SpeI(μL)	0.5
Buffer 2(μL)	2
(×10)BSA(μL)	2
dH₂O(μL)	3
TOTAL(μL)	20

--->Gel Check

Sample	left	right
Sample DNA(μL)	5	5
Loading Dye(μL)	1	1
TOTAL(μL)	6	6

Left side:BBa_R0010 Sample No.1

Right side:BBa_R0010 Sample No.2

-->(8/9)

Team:Output

Cloning Digestion

vector:BBa_R0040(1)75ng/μl
insert:BBa_S03158(2) 33.6ng/μl
insert:BBa_S03156(3) 33.6ng/μl
insert:Ba_S03160(4) 38.4ng/μl
vector:BBa_R0010(5) 100ng/μl
insert:BBa_J52008(6) 33.6ng/μl

Sample No.	1	2	3	4	5	6
DNA(digested sample)(μL)	50	54	54	45	14	54
SpeI(μL)	1	0	0	0	1	0
PstI(μL)	0.5	0.5	0.5	0.5	0.5	0.5
XbaI(μL)	0	0.5	0.5	0.5	0	0.5
BSA(x10)(μL)	0.6	7	7	6	2	7
Buffer2(μL)	6	-	-	-	2	-
Buffer3(μL)	-	7	7	6	-	7
dH₂O(μL)	1.9	1	1	2	0.5	1
TOTAL(μL)	60μl	70μl	70μl	60μl	20μl	70μl

-->left at for 3 hours at 37 degrees.

-->Gel Extraction

Sample No.	1	2	3	4	5	6
DNA(digested sample)(μL)	6	10	10	10	4	10
Loading Dye(μL)	2	2	2	2	1	2
dH₂O(μL)	4	-	-	-	1	2
TOTAL(μL)	12	12	12	12	6	12
well	10	7	7	6	5	7

-->DNA purification (1~4,6:7μl 5:6μl)

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