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Team:Chiba/Calendar-Home/7 September 2008
From 2008.igem.org
6 September 2008 <|> 8 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(6/9) Digestion Test
- Sample DNA 1 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](unknown)
- Sample DNA 2 : [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](100ng/μL)
| Sample No. | 1,2 |
| Sample DNA(μL) | 12 |
| PstI(μL) | 0.5 |
| SpeI(μL) | 0.5 |
| Buffer 2(μL) | 2 |
| (×10)BSA(μL) | 2 |
| dH2O(μL) | 3 |
| TOTAL(μL) | 20 |
- --->Gel Check
|
|
-->(8/9)
Team:Output
Cloning Digestion
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](1)75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2) 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](3) 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](4) 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](5) 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](6) 33.6ng/μl
| Sample No. | 1 | 2 | 3 | 4 | 5 | 6 | |
| DNA(digested sample)(μL) | 50 | 54 | 54 | 45 | 14 | 54 | |
| SpeI(μL) | 1 | 0 | 0 | 0 | 1 | 0 | |
| PstI(μL) | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | |
| XbaI(μL) | 0 | 0.5 | 0.5 | 0.5 | 0 | 0.5 | |
| BSA(x10)(μL) | 0.6 | 7 | 7 | 6 | 2 | 7 | |
| Buffer2(μL) | 6 | - | - | - | 2 | - | |
| Buffer3(μL) | - | 7 | 7 | 6 | - | 7 | |
| dH2O(μL) | 1.9 | 1 | 1 | 2 | 0.5 | 1 | |
| TOTAL(μL) | 60μl | 70μl | 70μl | 60μl | 20μl | 70μl |
-->left at for 3 hours at 37 degrees.
| Sample No. | 1 | 2 | 3 | 4 | 5 | 6 |
| DNA(digested sample)(μL) | 6 | 10 | 10 | 10 | 4 | 10 |
| Loading Dye(μL) | 2 | 2 | 2 | 2 | 1 | 2 |
| dH2O(μL) | 4 | - | - | - | 1 | 2 |
| TOTAL(μL) | 12 | 12 | 12 | 12 | 6 | 12 |
| well | 10 | 7 | 7 | 6 | 5 | 7 |
-->DNA purification (1~4,6:7μl 5:6μl)
