FIFO
|
Construction
| Description
| Validation Date
|
pFliL(RBS WT)-ECFP tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is ECFP fused with a LVA tag.
| October 24
|
pFliL(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFliL is GFP.
| October 24
|
pFlgA(+ RBS WT) - YFP Tripart (LVA+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a LVA tag.
| August 22
|
pFlgA(+ RBS WT) - YFP Tripart (LVA-)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused without a LVA tag.
| August 22
|
pFlgA(+ RBS WT)-YFP tripart (AAV+)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is EYFP fused with a AAV tag.
| October 24
|
pFlgA(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlgA is GFP.
| October 24
|
pFlhB(+ RBS WT)-mRFP tripart (+LVA)
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is mRFP with a LVA tag.
| October 24
|
pFlhB(+ RBS WT)-GFP
| Natural Promoter of E. coli regulated by FlhDC and FliA. The natural rbs of the downstream gene is conserved for a more natural expression of the following gene. The fluorescent marker associated with pFlhB is GFP.
| October 26
|
OSCILLATION
|
pFliL(+ RBS WT)-EnvZ*
| pFliL is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
| October 26
|
pFliL(+ RBS WT)-EnvZ*-GFP generator
| The same construction as before but with a GFP in order to follow the dynamic of expression of pFliL.
| October 29
|
pFlhB(+ RBS WT)-EnvZ*
| pFlhB is regulated by FlhDC and EnvZ regulates pFlhDC thanks to a negative feedback loop, origin of an oscillation behavior.
| October 24
|
pFlhB(+ RBS WT)-EnvZ*-GFP generator
| The same construction as before but with a GFP in order to follow the dynamic of expression of pFlhB.
| October 29
|
araC/pBad-EnvZ*
| A construction made for characterizing the repression power of the mutated EnvZ on our system
| October 27
|
SYNCHRONISATION
|
pLas - gfp Tripart
| Construction for characterization of the pLas promoter
| August 1
|
pLas - ECFP
| The same as before but with another fluorophore
| August 1
|
strongest promoter-rbs-lasR-dble ter
| A construction for a strong constitutive expression of the Las Regulator in order to get maximal expression in presence of AHL
| August 20
|
Strong promoter-rbs-lasR-dble ter
| A derived construction of the previous one for smaller constitutive expression of LasR in the Bacteria.
| August 20
|
Strongest RBS (1)- LasR activator (+LVA)
| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
| August 13
|
rbs-lasR + dbl ter
| Intermediary construction. We uses different rbs for Las R in order to get various response dynamic to the presence of AHL
| August 19
|
lasR activator + LVA - Double Terminator
| Intermediary construction
| August 1
|
RBS lasI - GFP tripart
| Construction that has to be associated with the FIFO. We will know when LasI is expressed and then when AHL is produced.
| August 13
|
RBS-lasI - ECFP
| The same as the previous one but with another fluorophore.
| August 13
|
NEW GENE AND PROMOTER BIOBRICKS
|
pFliA
| Natural promoter of E. coli, extracted by PCR and then inserted in a biobrick vector. pFiA is regulated by FlhDC.
| October 04
|
pFliL (+RBS WT)
|
| October 10
|
pFlgA (+RBS WT)
|
| October 10
|
pFlgB (+RBS WT)
|
| October 10
|
pFlhB (+RBS WT)
|
| October 10
|
EnvZ*
|
| September 04
|
FliA
|
| September 04
|
FlhDC
|
| August 13
|