Alberta NINT/3 July 2008
From 2008.igem.org
lab work (SD):
Sequenced pSB4A5 to verify. Made overnights from four colonies from each plate (K102022 and K102032) from 02/07/08.
lab work (JD):
From sequencing K102006.4 becomes official K102006 K102007.5 becomes official K102007 Digested pSB4A5 with EcoR1 and Pst1 Digested K102006 and K102007 with EcoR1 and Pst1 Ran 2% agarose gel electrophoresis Made glycerol stock of K102006 and K102007