Team:KULeuven/Project/Reset
From 2008.igem.org
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Reset
figuur
The reset mechanism is made to destroy all HSL (link) at the time, when input re-emerges. Originally, a pulse of lactonase (figuur) was thought to do the thing, but modeling proved that the amount of lactonase was not enough. This simplified the device a lot.
The aiiA gene (BBa_C0060) codes for lactonase, which breaks down HSL. It is regulated by the output signal from the filter, so that background input signal does not result in timer reset.