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From 2008.igem.org

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock) PCR products PCR products Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date Plasmids: MiniPrep Products Plasmids: MiniPrep Products 2008 Number Name Biobricks Description Products in use Date MP100 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1) July 26 MP101 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter July 22July 23July 26 MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter July 22July 26 MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated) July 23July 26 MP104 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter July 23July 26 MP105 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI July 26 MP106 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR July 26 MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag July 23 MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator July 22July 23July 26 MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP July 26 MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP July 23July 26 MP111 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP July 23July 26 MP112 OmpR M571 July 23 MP113 EnvZ I2815 July 23 MP114 [http://partsregistry.org/Part:BBa_C0040 C0040] tetR repressor July 23 MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 July 26 MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 July 24July 26 MP118 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Double terminator July 24July 26 MP119 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD July 24July 26 MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Strong RBS (Efficiency = 0,6) July 26 MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term) July 24July 26 MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] gfp tri-part; strong rbs July 26 Digestion Products (Legend: Vector, Insert) Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product Left []ng/µL Date D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D100.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26 D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29 D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26 D102.2 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 29 D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D103.2 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2100 883 10 July 26 D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D104.2 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26 D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26 D105.2 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 29 D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26 D106.2 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 5 July 29 D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 5 July 26 D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 26 D108.2 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29 D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 5 July 26 D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 20 July 26 D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26 D111.2 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 29 D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2756 726 20 July 26 D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26 D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26 D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D115.2 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29 D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D116.1 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29 D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29 D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26 D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26 D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26 D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26 D122 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26 D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D123.2 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 10 July 26 D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D124.2 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26 D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26 D125.2 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 15 July 26 D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D126.2 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26 D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D127.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29 D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D129.2 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29 D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.2 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D130.3 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26 D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.2 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 D131.3 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 10 July 26 Strains (glycerol stock) Strains glycerol stock Number Name Description Verified clone Date S103 [http://partsregistry.org/Part:BBa_I0500 I0500] AraC pBAD July 23 S106 [http://partsregistry.org/Part:BBa_E0422 E0422] ECFP (RBS+LVA+Term) July 23 S107 [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS (Efficiency = 1) July 23 S108 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated) July 23 S109 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter July 23 S110 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter July 23 S111 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter July 23 S112 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI July 23 S113 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR July 23 S114 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag July 23 S115 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator July 23 S116 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP July 23 S117 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP July 23 S118 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP July 23 S119 OmpR M571 July 23 S120 EnvZ I2815 July 23 S121 [http://partsregistry.org/Part:BBa_C0040 C0040] tetR repressor July 23 S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] July 23 S123 L101 D110 (BV) rbs - Tet R & D131 (BI) gfp Tripart S124 L102 D129 (BV) Strong rbs & D118 (BI) yfp S125 L103 D129 (BV) Strong rbs & D122 (BI) gfp S126 L104 D129 (BV) Strong rbs & D114 (BI) las R activator S127 L105 D123 (BV) Strong promoter & D130 (BI) ECFP S128 L107 D103 (BV) Strongest promoter & D131 (BI) ECFP S129 L109 D124 (BV) Strong promoter & D130 (BI) ECFP S130 L110 D124 (BV) & D130 (BI) S131 L111 D104 (BV) Weak promoter & D130 (BI) ECFP S132 L112 D104 (BV) Weak promoter & D131 (BI) gfp S133 L113 D126 (BV) Arac pBAD & D130 (BI) ECFP S134 L114 D126 (BV) Arac pBAD & D131 (BI) gfp Tripart

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