Team:ESBS-Strasbourg/Protocols

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Contents

1 E.Coli
2 Cloning
3 Solutions and buffers
4 Fluorescence

E.Coli

Preparation of competent cells (Mariels/Barbaras Protocol) Competent Bacteria

Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]

Transformation of competent cells (Mariels/Barbaras Protocol) Transformation

Transformation of competent cells with heat shock Transformation HS

Cloning

Solutions and buffers

SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]
SOC for bacterial transformation purposes [http://openwetware.org/wiki/SOC SOC]
TENS for the do it yourself MiniPrep TENS

Fluorescence

Further source for protocols: [http://www.protocol-online.org/ Protocol-online.org]

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