Team:KULeuven/26 August 2008

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  KULeuven iGEM 2008 project Biological robotics

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Lab Work

Wet Lab

Looks like we've finally nailed the transduction-PCR. The photo of our gel looks very promissing, tomorrow we'll discuss it with our advisors, but we think our cells should be fine!

We cut [http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610] with XbaI and EcoRI. There is something wrong with R0011+F1610, so we have to do this again. We can't see anything on the gel with the digests. We also had a lot of troubles with cutting F1610. We think that something might be wrong with this part.

The following parts were electroporated: [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] and [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2].

The ligations that were electroporated yesterday, gave colonies ([http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]). We did a PCR to test these ligations. We are not sure of the ligations J23116+B0032 and R0040+J23022. The other ones seem to be OK and we made a liquid culture of them.

Dry Lab

General meeting with the team decided upon some important/emergency building-stuff :) So, here we go. The two parallel schemes for building the system:

new key-lock

old key-lock, parallel scheme

Ethics

We've started on thinking about the ethical part of our project. We would start from the film "I, Robot" and then focus further on the laws of robotics. Benjamien and Hanne did a lot of research on probiotics, ethics and genetic engineering.

Modeling

Some more stochastic stuff

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