Team:Hawaii/Notebook/2008-08-26

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Things we did today

Wetlab work

Ran RE digests on gel

Grace
  • Gel did not resolve. Bands were convex, poor separation between bands. Bad buffer?
  • Redid RE digests from yesterday
  • Used PCR products of nir and J33207

Inoculated for plasmid prep

Grace
  • BB-pRL1383a and J33207 in TB+amp100

Gel Purified

Krystle
  • Ran overnight ligation products on a 3% agarose gel
discussion: If we decide to do the 3A in two parts, we will have to re-restriction digest the first part before moving on

Transformation of ligated GFPf + TT

Krystle
  • Performed transformation protocol with DB3.1 and GFPf + tt ligation from 08/25

Ligation

Margaret

  • Overnight in 4°C
  • rep+pSB1A3 (1:1)
  • rep+pSB1A3 (1:3)
  • rep+pSB1A3 (1:6)
  • P1 lytic +pSB1A3 (1:1)
  • P1 lytic +pSB1A3 (1:3)
  • P1 lytic +pSB1A3 (1:6)
  • [Plac B0030]+pSB1A3 (1:3)
  • [Plac B0030]+pSB1A3 (1:6)
  • [Plac B0034]+pSB1A3 (1:6)**ran out of vector

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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