Team:KULeuven/28 August 2008

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• The Team
  Research Labs and Groups Students Instructors Advisors Pictures
• The Project
  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
• Ethics
  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

  Concerns & Issues

  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

  Afterword References
• Submitted Parts
  Sandbox
• Modeling
  Overview Kinetic Constants Components

  Output Filter InverTimer Reset Cell Death Memory

  Full Model Sensitivity Analysis Multi-cell Model Diffusion
• Data Analysis
  Overview New Parts

  GFP (LVA-tag) Other

  Components

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• Software
  Multi-cell Toolbox Simbiology2LaTeX Toolbox
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  Lab Data

  Freezer Primers Ligation

  Tools Press Acknowledgments

Lab Work

Wet Lab

• The ligations with B0015 (C0012+B0015, C0060+B0015, C0040+B0015, K145001+B0015, K145151+B0015, K45015+B0015) were electroporated again, but now they were plated on an ampicillin plate.
• A glycerol stock was made of the succeeded ligations.
• The new parts arrived today. They were also plated.
• The ligations of K145001 and K145151 with pSB1A2 were MiniPrepped and then we did a PCR to test the ligation.
• The digests of R0011, R0040+J23022, C0062 and B0033 were put on gel and purified.
• Some more digests were made, but this time we cut with SpeI, XbaI and PstI. We cut B0015 as insert and C0060, K145015, C0012 and C0040 were cut as vector.

Dry Lab

We did some more research on bioethics and human practice.

Modeling

Great Succes: we succeeded in modeling cell-multiplication in a Matlab-environment. This will be our starting point for modeling cell division and work in a multi-cell environment! [we did what the lab couldn't do]

Parameter checking has almost reached its final state.

Simulations of the different parts has continued.

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