Team:KULeuven/20 August 2008

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• The Team
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  Summary Components

  Input Output Filter InverTimer Reset Cell Death Memory

  Safety End Evaluation Literature Brainstorm
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  Introduction Three A's

  Defining the three A's Developments in modern physics Developments in biology

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  Defining synthetic biology A framework Concerns Issues The need for answers

  Biological Robotics

  KULeuven iGEM 2008 project Biological robotics

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  Output Filter InverTimer Reset Cell Death Memory

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Lab Work

Wet Lab

• Ligations C0060+B0015, C0040+B0015, C0012+B0015 and K145151+B0015 were miniprepped, but were mixed up, so have to be redone. They are cut with XbaI anyway. Colonies were re-inoculated in liquid medium to grow overnight and to be miniprepped again tomorrow.
• We tried to put K145001 (T7 polymerase without tag) into a vector using TOPO TA cloning.
• We performed a PCR on the Klenow mix of the end-filling (part K145150). There were no bands, so it failed. We also tried to digest this mixture and ligate it into a pSB1A2 vector (without purifying the mixtures first). Hopefully we'll have colonies tomorrow.
• We set up some ligations: J23116+B0032, R0040+B0032, R0011+F1610 and K145151+pSB1A2.

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