Team:KULeuven/Freezer

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Miniprep (stored in the fridge in the lab at 8°C)

MiniPrep Products: Parts

Part	Plasmid	Description	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 10	112.3	7	July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 20	130.3	8	July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	303.1	9	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 20	213.6	10	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 48	311.0	11	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 30	214.0	12	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	209.5	13	July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Terminator	+/- 40	63.0	6	July 23
[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	RBS(medium)	+/- 5	51.0	57	August 11
[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	RBS(medium)	+/- 20	47.2	55	August 27
[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	RBS(medium)	+/- 40	27.0	56	August 27
[http://partsregistry.org/Part:BBa_B0033 B0033]	pSB1AK3	RBS	+/- 50	36.5	43	August 27
[http://partsregistry.org/Part:BBa_B0033 B0033]	pSB1AK3	RBS	+/- 23	38.4	44	August 27
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 20	73.8	47	July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 30	95.1	48	July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	RBS (efficiency 1)	+/- 40	104.0	49	July 29
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1AK3	RBS	+/- 15	57	50	August 27
[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1AK3	RBS	+/- 50	29.6	51	August 27
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI	+/- 30	133.1	20	July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI repressor	+/- 40	146.4	22	July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]	pSB1A2	LacI repressor	+/- 40	203.1	21	July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]	pSB1A2	TetR repressor	+/- 20	177.1	4	July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]	pSB1A2	TetR repressor	+/- 30	161.7	5	July 24
[http://partsregistry.org/Part:BBa_C0060 C0060]	pSB1A2	lactonase production	+/- 48	84.2	53	August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]	pSB1A2	LuxR repressor	+/- 48	76.2	38	August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]	pSB1AK3	LuxR repressor	+/- 25	41.5	36	August 27
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	HSL Sender Device	+/- 50	57.6	16	August 12
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	HSL Sender Device	+/- 40	53.1	17	August 12
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	HSL Sender Device	+/- 50	41.4	18	August 27
[http://partsregistry.org/Part:BBa_F1610 F1610]	pSB1AK3	HSL Sender Device	+/- 20	31.5	19	August 27
[http://partsregistry.org/Part:BBa_I712022 I712022]	BBa_J52017 (Km, Ap)	T7 polymerase	+/- 40	394.1	35	July 31
[http://partsregistry.org/Part:BBa_I712074 I712074]	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 42.5	208.2	32	July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 45	20.5	33	August 27
[http://partsregistry.org/Part:BBa_I712074 I712074]	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 45	63.9	34	August 27
[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	3d key+ terminator	+/- 40	160.7	26	July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	3d key+ terminator	+/- 46	308.3	27	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 35	117.4	23	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 39.5	144.8	24	July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	3d lock	+/- 48	129.1	25	July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 46	375.1	28	July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	99.2	30	August 27
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	69.2	31	August 27
[http://partsregistry.org/Part:BBa_J23109 J23109]	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	404	29	July 29
[http://partsregistry.org/Part:BBa_J23116 J23116]	BBa_J61002 (Ap)	Constitutive promoter	+/- 48	328.8	52	August 08
[http://partsregistry.org/Part:BBa_R0011 R0011]	pSB1A2	LacI regulated promoter	+/- 40	69.8	42	July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]	pSB1AK3	LacI regulated promoter	+/- 50	30.8	40	August 27
[http://partsregistry.org/Part:BBa_R0040 R0040]	pSB1A2	tetR repressible promoter	+/- 48	37.9	46	August 11
[http://partsregistry.org/Part:BBa_R0062 R0062]	pSB1A2	LuxR regulated promoter	+/- 40	104.1	3	July 31
[http://partsregistry.org/Part:BBa_R0084 R0084]	pSB1A2	OmpF promoter	+/- 40	32.0	14	July 23
[http://partsregistry.org/Part:BBa_R1052 R1052]	pSB1K0	434cI regulated promoter	+/- 15	27.5	60	Sept 05
[http://partsregistry.org/Part:BBa_C0060 C0060]	pSB1A2	Lactonase	30	155.3	54	July 31
[http://partsregistry.org/Part:BBa_C0062 C0062]	pSB1A2	LuxR repressor/activator	?	159.4	35	July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]	pSB1A2	lacI + pL promoter	?	68.0	39	July 31
[http://partsregistry.org/Part:BBa_J23066 J23066]	J23006	ribokey	+/- 45	541	58	August 30
[http://partsregistry.org/Part:BBa_P0353 P0353]	pSB1AK3	cII p22 van memory	+/- 45	406.9	1	August 30
[http://partsregistry.org/Part:BBa_P0152 P0152]	pSB1A2	cI 434 van memory	+/- 44	265.4	2	August 30
[http://partsregistry.org/Part:BBa_E0240 E0240]	pSB1A2	GFP generator	+/- 44	281	59	August 30
[http://partsregistry.org/Part:BBa_C0056 C0056]	pSB1A2	cI represor	+/- 45	419.8	64	August 30
[http://partsregistry.org/Part:BBa_C0061 C0061]	pSB1A2	autoinducer ASL synthetase, LuxI	+/- 45	338.6	65	August 30
[http://partsregistry.org/Part:BBa_B0014 B0014]	pSB1AK3	terminator	+/- 45	344.4	66	August 30
[http://partsregistry.org/Part:BBa_E0040 E0040]	pSB1A2	GFP	+/- 48	305.8	67	August 30
[http://partsregistry.org/Part:BBa_R0053 R0053]	pSB1A2	p22 cII regulated promotor	+/- 25	72.1	69	August 30
[http://partsregistry.org/Part:BBa_I714074 I714074]	pSB1A2	ribokey	+/- 48	469	68	August 30
[http://partsregistry.org/Part:BBa_R0040 R0040]	pSB1A2	tetR repressible promotor	+/- 27	74.6	45	Sept 01

MiniPrep Products: Ligations

Parts	Plasmid	Description	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Cell Death	+/- 46	124.8	A1	August 08
[http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Memory	+/- 46	276.7	D5	Sept 05
[http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Inverter	+/- 46	159.1	D6	Sept 05
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240]	pSB1A2	Ligation for Output	+/- 46	281.9	D7	Sept 05
[http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033]	pSB1A2	Ligation for Cell Death	+/- 46	118.1	D8	Sept 05
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]	J23006 (Ap)	Ligation for Filter (new)	+/- 46	421.9	D9	Sept 05
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033]	pSB1A2	Ligation for Memory	+/- 46	81.2	E1	Sept 05
[http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152]	pSB1A2	Ligation for Memory	+/- 46	109.5	E2	Sept 05
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1AK3	Ligation for Output	+/- 46	151.8	A5	August 08
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	89.9	A3	August 08
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	94.4	A4	August 08
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Input	+/- 40	102.5	A8	August 25
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Input	+/- 35	96.2	B2	August 25
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	GFP with LVA tag	+/- 46	152.7	A2	August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	GFP+LVA	+/- 48	257	E4	August 30
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB	+/- 48	204.6	E3	August 30
[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	T7 without UmuD	+/- 48	414.8	E5	August 30
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB	+/- 48	188.4	E6	August 30
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB coding region	+/- 46	57.8	B5	August 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB coding region	+/- 46	72.1	B8	August 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB coding region	+/- 46	18.5	B7	August 28
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	ccdB coding region	+/- 46	23.3	B6	August 28
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]	pSB1A2	GFP with LVA tag	+/- 20	104.5	F6	August 08
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for output	+/- 30	64.4	A9	August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Output	+/- 35	93.1	B1	August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 35	81.3	B3	August 27
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 35	86.3	B4	August 27
[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Inverter	+/- 40	226.9	C1	Sept 01
[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Input	+/- 40	91.6	C2	Sept 01
[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Input	+/- 40	143.8	C3	Sept 01
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Cell Death	+/- 40	122.8	C4	Sept 01
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Cell Death	+/- 40	119.8	C5	Sept 01
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Output	+/- 40	175.5	C7	Sept 01
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Reset	+/- 40	174.2	C8	Sept 01
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Reset	+/- 40	188.5	C9	Sept 01
[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Filter	+/- 40	242.9	D1	Sept 01
[http://partsregistry.org/Part:BBa_K145201 K145201]	pSB1A2	Input: complete	+/- 40	193.5	E7	Sept 06
[http://partsregistry.org/Part:BBa_K145278 K145278]	pSB1A2	part for filter	+/- 42	185.2	G4	Sept 12
[http://partsregistry.org/Part:BBa_K145201 K145201]	pSB1A2	Input: complete	+/- 40	200.7	E8	Sept 06
[http://partsregistry.org/Part:BBa_K145253 K145253]	pSB1A2	Inverter (old) - part 1	+/- 40	223.3	E9	Sept 06
[http://partsregistry.org/Part:BBa_K145253 K145253]	pSB1A2	Inverter (old) - part 1	+/- 40	202.9	F1	Sept 06
[http://partsregistry.org/Part:BBa_K145251 K145251]	pSB1A2	Reset (old): complete	+/- 40	168.6	F2	Sept 06
[http://partsregistry.org/Part:BBa_K145251 K145251]	pSB1A2	Reset (old): complete	+/- 40	154.5	F3	Sept 06
[http://partsregistry.org/Part:BBa_K145205 K145205]	pSB1A2	Output: complete	+/- 40	227.4	F4	Sept 06
[http://partsregistry.org/Part:BBa_K145205 K145205]	pSB1A2	Output: complete	+/- 40	215.2	F5	Sept 06
[http://partsregistry.org/Part:BBa_K145254 K145254]	pSB1A2	Ligation for Invertimer	+/- 40	137.6	H3	Sept 9
[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Filter	+/- 40	183.1	D2	Sept 01
[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Inverter	+/- 40	56.0	D3	Sept 01
[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]	pSB1A2	Ligation for Inverter	+/- 40	106.3	D4	Sept 01
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Inverter	+/- 40	158.6	B9	Sept 01
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Ligation for Inverter	+/- 40	168.6	G5	Sept 9
[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 BE0240]	pSB1A2	Control for K145150 promotor	+/- 40	178.4	G6	Sept 9
[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	pSB1A2	Hybrid Promotor	+/- 40	97.6	G7	Sept 9
[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	pSB1A2	Ligation for Memory	+/- 40	89	G8	Sept 9
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2	Celldeath	+/- 40	122.8	G9	Sept 9
[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_B0034 B0034]	pSB1A2	Ligation for Celldeath	+/- 30	47.5	H1	Sept 9
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353]	pSB1AK3	Ligation for Memory	+/- 10	229.1	H2	Sept 9
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 20	150.2	A6	August 20
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 20	58.3	A7	August 20
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	BBa_J23006 (Ap)	Ligation for Filter (new key)	+/- 45	394.3	G3	Sept 12
[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_B0015 B0015]	pSB1A2 (Ap)	Ligation for Memory	+/- 50	141.3	H8	Sept 13
[http://partsregistry.org/Part:BBa_K145272 K145272]	pSB1A2 (Ap)	Ligation for Filter test	+/- 50	145.9	H9	Sept 13

Digests (stored in the fridge in the lab at 8°C)

Restriction Digests: parts

Part	Enzyme 1	Enzyme 2	vector/insert	length (bp)	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_B0034 B0034]	EcoR I	Xba I	Vector	2075	25	-	58	September 11
[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3303	11	11.7	1	July 31
[http://partsregistry.org/Part:BBa_B0015 B0015]	Pst I	Xba I	Insert	144	25	3.8	3	Sept 09
[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3303	10	13.6	2	August 26
[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Xba I	Vector	2092	5	17.6	25	August 19
[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Xba I	Vector	2092	20	6.4	24	August 29
[http://partsregistry.org/Part:BBa_B0033 B0033]	EcoR I	Xba I	Vector	2092	20	10.9	31	August 27
[http://partsregistry.org/Part:BBa_B0014 B0014]	EcoR I	Spe I	Insert	110	20	1.3	50	Sept 02
[http://partsregistry.org/Part:BBa_C0040 C0040]	EcoR I	Spe I	Insert	677	18	7.1	6	July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]	Spe I	Pst I	Vector	3192	25	16.4	32	August 29
[http://partsregistry.org/Part:BBa_C0060 C0060]	Spe I	Pst I	Vector	2853	25	16.3	18	August 29
[http://partsregistry.org/Part:BBa_C0040 C0040]	Spe I	Pst I	Vector	2724	25	19.2	7	August 29
[http://partsregistry.org/Part:BBa_E0040 E0040]	Spe I	Pst I	Vector	2799	25	28.4	55	Sept 06
[http://partsregistry.org/Part:BBa_K145015 K145015]	Spe I	Pst I	Vector	2823	25	18.8	33	August 29
[http://partsregistry.org/Part:BBa_K145151 K145151]	Spe I	Pst I	Vector	2370	25	14.5	34	August 29
[http://partsregistry.org/Part:BBa_K145151 K145151]	Spe I	EcoR I	Vector	2370	25	25.2	49	August 29
[http://partsregistry.org/Part:BBa_K145001 K145001]	Spe I	Pst I	Vector	4782	20	6.2	40	August 29
[http://partsregistry.org/Part:BBa_C0061 C0061]	Spe I	Pst I	Vector	2680	20	17.5	39	August 31
[http://partsregistry.org/Part:BBa_C0056 C0056]	Spe I	Pst I	Vector	2700	20	19.0	36	August 31
[http://partsregistry.org/Part:BBa_C0060 C0060]	EcoR I	Spe I	Insert	803	28	5.4	17	August 12
[http://partsregistry.org/Part:BBa_C0060 C0060]	EcoR I	Spe I	Insert	806	9	8.2	16	August 01
[http://partsregistry.org/Part:BBa_C0062 C0062]	EcoR I	Spe I	Insert	774	18	24.7	11	August 01
[http://partsregistry.org/Part:BBa_C0062 C0062]	EcoR I	Spe I	Insert	774	25	8.3	12	August 27
[http://partsregistry.org/Part:BBa_C0062 C0062]	EcoR I	Spe I	Insert	774	28	5.0	10	August 12
[http://partsregistry.org/Part:BBa_C0062 C0062]	Pst I	Xba I	Insert	771	40	?	56	Sept 10
[http://partsregistry.org/Part:BBa_F1610 F1610]	EcoR I	Xba I	Vector	3956	26	4.5	30	August 19
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3090	2	12.9	20	July 30
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3090	45	1.3	21	July 31
[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3308	25	5.1	22	August 07
[http://partsregistry.org/Part:BBa_J23100 J23100]	EcoR I	Spe I	Insert	52	38.1	2.6	5	July 24
[http://partsregistry.org/Part:BBa_J23109 J23109]	EcoR I	Spe I	Insert	52	21	4.2	29	July 29
[http://partsregistry.org/Part:BBa_J23116 J23116]	EcoR I	Spe I	Insert	52	+/-20	8.1	26	August 12
[http://partsregistry.org/Part:BBa_K145001 K145001]	EcoR I	Spe I	Insert	2732	+/-8	14.1	41	August 12
[http://partsregistry.org/Part:BBa_K145013 K145013]	EcoR I	Spe I	Insert	660	+/-25	20.8	53	Sept 05
[http://partsregistry.org/Part:BBa_K145150 K145150]	EcoR I	Spe I	Insert	110	+/-25	9.2	54	Sept 05
[http://partsregistry.org/Part:BBa_K145151 K145151]	Xba I	/	Vector	2400	28	?	57	August 12
[http://partsregistry.org/Part:BBa_K145001 K145001]	EcoR I	Spe I	Insert	2732	+/-8	24.3	48	August 12
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2]	EcoR I	Spe I	Plasmid	2079	9	18.2	46	August 01
[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoR I	Spe I	Plasmid	2056	24	27.7	47	August 25
[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	EcoR I	Spe I	Plasmid	2056	24	24.3	52	Sept 05
[http://partsregistry.org/Part:BBa_R0011 R0011]	EcoR I	Spe I	Insert	72	20	8.5	13	July 30
[http://partsregistry.org/Part:BBa_E0240 E0240]	EcoR I	Xba I	Vector	2955	28	25.2	42	Sept 01
[http://partsregistry.org/Part:BBa_E0040 E0040]	EcoR I	Spe I	Insert	720	28	8.3	37	Sept 01
[http://partsregistry.org/Part:BBa_C0056 C0056]	EcoR I	Spe I	Insert	636	23	10.1	35	Sept 01
[http://partsregistry.org/Part:BBa_C0061 C0061]	EcoR I	Spe I	Insert	618	28	9.0	38	Sept 01
[http://partsregistry.org/Part:BBa_P0152 P0152]	EcoR I	Xba I	Vector	2930	28	23.2	43	Sept 01
[http://partsregistry.org/Part:BBa_P0353 P0353]	EcoR I	Xba I	Vector	4000	28	15.3	45	Sept 01
[http://partsregistry.org/Part:BBa_J23066 J23066]	EcoR I	XbaI	Vector	2700	28	11.4	27	Sept 01
[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3303	20	12.6	4	Sept 01
[http://partsregistry.org/Part:BBa_B0034 B0034]	EcoR I	Xba I	Vector	2091	28	15.6	44	Sept1
[http://partsregistry.org/Part:BBa_R0011 R0011]	EcoR I	Spe I	Insert	72	20	7.5	14	July 30
[http://partsregistry.org/Part:BBa_R0011 R0011]	EcoR I	Spe I	Insert	72	25	2.1	15	August 27
[http://partsregistry.org/Part:BBa_R0062 R0062]	EcoR I	Spe I	Insert	55	27	7.8	23	August 07
[http://partsregistry.org/Part:BBa_R0084 R0084]	EcoR I	Spe I	Insert	125	33.4	3.8	8	July 24
[http://partsregistry.org/Part:BBa_R0084 R0084]	EcoR I	Spe I	Insert	125	25	6.1	9	July 29
[http://partsregistry.org/Part:BBa_R0053 R0053]	EcoR I	Spe I	Insert	70	20	1.1	51	Sept 02
[http://partsregistry.org/Part:BBa_I712074 I712074]	EcoR I	Spe I	Insert	61	25	3.4	28	July 29
[http://partsregistry.org/Part:BBa_J23078 J23078]	Xba I	Pst I	Insert	100	30	7.8	59	Sept 13
[http://partsregistry.org/Part:BBa_C0062 C0062]	Pst I	Spe I	Vector	2820	30	8.0	60	Sept 13

Restriction Digests: Ligations

Part	Enzyme 1	Enzyme 2	vector/insert	length (bp)	µl left	ng/µl	Box	Date
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Xba I	Vector	3350	+/-30	-	F3	September 11
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	4080	+/-30	32.8	A1	August 14
[http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2800	+/-30	17.3	A2	Sept 06
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]	EcoR I	Spe I	Insert	411	+/-30	6.6	A3	Sept 06
[http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P0152 P0152]	EcoR I	Spe I	Insert	926	+/-30	9.0	A4	Sept 06
[http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033]	EcoR I	Spe I	Insert	132	+/-30	6.1	A5	Sept 06
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033]	EcoR I	Spe I	Insert	86	+/-30	4.1	A6	Sept 06
[http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3930	+/-30	12.8	A7	Sept 06
[http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	4915	+/-30	13.7	A8	Sept 06
[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_B0034 B0034]	SpeI	Pst I	Vector	2682	+/-30	?	D5	Sept 10
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240]	EcoR I	Xba I	Vector	2981	+/-30	14.8	A9	Sept 06
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	4086	+/-30	14.8	B1	August 14
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Spe I	Insert	112	+/-30	3.9	B2	August 14
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Spe I	Insert	101	+/-30	4.0	B3	August 14
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2986	+/-25	17.8	B4	Sept 03
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2857	+/-25	18.7	B5	Sept 03
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2956	+/-25	20.8	B6	Sept 03
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2505	+/-25	18.1	B7	Sept 03
[http://partsregistry.org/Part:BBa_K145201 K145201]	EcoR I	Spe I	Insert	900	+/-10	7.3	D1	Sept 07
[http://partsregistry.org/Part:BBa_K145205 K145205]	EcoR I	Xba I	Vector	3065	+/-20	26.9	D2	Sept 07
[http://partsregistry.org/Part:BBa_K145253 K145253]	EcoR I	Spe I	Insert	1410	+/-20	10.9	D3	Sept 07
[http://partsregistry.org/Part:BBa_K145251 K145251]	EcoR I	xba I	Vector	3133	+/-20	21.0	D4	Sept 07
[http://partsregistry.org/Part:BBa_K145258 K145258]	EcoR I	Spe I	Insert	852	+/-30	-	F4	Sept 11
[http://partsregistry.org/Part:BBa_K145254 K145254]	EcoR I	Xba I	Vector	2925	+/-30	?	D7	Sept 10
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2505	+/-30	?	D9	Sept 10
[http://partsregistry.org/Part:BBa_K145150 K145150]+[http://partsregistry.org/Part:BBa_E0240 E0240]	EcoR I	Xba I	Vector	3004	+/-30	?	E1	Sept 10
[http://partsregistry.org/Part:BBa_K145013 K145013]+[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]	Spe I	Pst I	Vector	2682	+/-30	?	E2	Sept 10
[http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	3327	+/-25	18.1	B8	Sept 03
[http://partsregistry.org/Part:BBa_E0040 E0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]	EcoR I	Xba I	Vector	2919	+/-30	?	D8	Sept 10
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	71	+/-28	2.5	B9	August 25
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	144	+/-20	0.9	C1	August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	144	+/-30	2.9	C2	August 21
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	90	+/-28	2.6	C3	August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_P0353 P0353]	EcoR I	Xba I	Vector	4100	+/-30	?	D6	Sept 10
[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	90	+/-25	3.8	C4	Sept 05
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]	EcoR I	Spe I	Insert	311	+/-28	3.6	C5	August 27
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]	EcoR I	Spe I	Insert	144	+/-28	2.4	C6	August 25
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]	EcoR I	Spe I	Insert	365	+/-20	6.9	C7	August 14
[http://partsregistry.org/Part:BBa_I712074 I712074]	Pst I	Spe I	Vector	3468	+/-30	7.9	5E	Sept 13
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]	EcoR I	Spe I	Insert	500	+/-30	6.9	6E	Sept 13

Things stored in freezer in lab (-20°C)

Miscellaneous

Description	Name	µl left	Concentration	Box	Date
Primer GFP-LVA	1084	90	20 µM	2	August 01
Primer GFP-LVA	1085	90	20 µM	4	August 01
dNTP mix	ATP, GTP, TTP, CTP	200	10 mM	81	August 28
Primer GFP-LVA	1084	500	100 µM	1	August 01
Primer GFP-LVA	1085	500	100 µM	3	August 01
Primer Test	1074	800	100 µM	5	August 07
Primer Test	1075	780	100 µM	7	August 07
Primer T7-UmuD	1076	560	100 µM	9	August 07
Primer T7-UmuD	1077	477	100 µM	11	August 07
Primer T7-UmuD	1078	467	100 µM	13	August 07
Primer T7-UmuD	1079	45	100 µM	15	August 07
Primer Transduction	1097	627	100 µM	17	August 07
Primer Transduction	1098	710	100 µM	19	August 07
Primer Transduction	1099	608	100 µM	21	August 07
Primer Anti-sense LuxI	1100	522	100 µM	23	August 07
Primer Anti-sense LuxI	1101	237	100 µM	25	August 07
Primer Test	1074	100	20 µM	6	August 07
Primer Test	1075	100	20 µM	8	August 07
Primer T7-UmuD	1076	100	20 µM	10	August 07
Primer T7-UmuD	1077	100	20 µM	12	August 07
Primer T7-UmuD	1078	100	20 µM	14	August 07
Primer T7-UmuD	1079	100	20 µM	16	August 07
Primer Transduction	1097	100	20 µM	18	August 07
Primer Transduction	1098	100	20 µM	20	August 07
Primer Transduction	1099	100	20 µM	22	August 07
Primer Anti-sense LuxI	1100	100	20 µM	24	August 07
Primer Anti-sense LuxI	1101	100	20 µM	26	August 07
dNTP mix	ATP, GTP, TTP, CTP	90	2 µM	80	August 19
Primer ccdB	1127	100	100 µM	27	August 19
Primer ccdB	1128	100	100 µM	29	August 19
Primer K145150	1129	100	100 µM	31	August 19
Primer K145150	1130	100	100 µM	34	August 19
Primer ccdB	1127	90	20 µM	28	August 19
Primer ccdB	1128	980	20 µM	30	August 19
Primer K145150	1129	990	1 µM	33	August 19
Primer K145150	1129	90	20 µM	32	August 19
Primer K145150	1130	990	1 µM	36	August 19
Primer K145150	1130	90	20 µM	35	August 19
Primer K145014 (2.1)	1156	990	100 µM	37	Sept 01
Primer K145014 (2.1)	1156	90	20 µM	38	Sept 01
Primer Suffix	1153	990	100 µM	39	Sept 02
Primer Suffix	1153	90	20 µM	40	Sept 02
Primer Prefix	1152	990	100 µM	41	Sept 02
Primer Prefix	1152	90	20 µM	42	Sept 02