Team:Hawaii/Notebook/2008-10-15
From 2008.igem.org
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Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Construction of secretion device (cont.)
- Grace
- Transformed DH5α
- Used 5 μl of n/p + rgt ligation reaction
- Used 1 μl of J33207/pRL1383a ligation reaction
- Plated 25 μl, 50 μl, 75 μl, and 100 μl plates in hopes of isolating single colonies
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]