Team:Hawaii/Notebook/2008-10-20

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Things we did today

Wetlab work

Large scale plasmid prep of BB-pRL1383a (blue)

Grace


PCR of J04430 (lac+rbs+GFP+tt+tt)

Grace
  • Will act as (-) control for GFP secretion in cyanos

Overnight RE digest

Grace
  • EcoRI and PstI digest of BBpRL1383a and J04430

Made LB+sp+sm plates for antibiotic gradient test on Wednesday

Grace <strong>
  • 30 ml plates

Discussion

Quote of the Day

Garbage truck dripping E. coli... this has got to stop. - Roswell Mayor, http://www.youtube.com/watch?v=f7mEb-p4F44 (ok maybe we should start taking Amp + Kan + Sm + Sp before going to the bathroom)


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